Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells

Jingzhu Lv, Xiaojie Zhang, Caizhi Wang, Hongtao Wang, Ting Wang, Zhongqing Qian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Preeclampsia (PE) is a pregnancy disorder with a high mortality rate. Patients with PE exhibit systemic high oxidative stress status and inflammatory immune activation. This study aims to define the role of H2O2 in the activation of neutrophils and T lymphocytes in PE patients. CD3+/HLA-DR+ cells in blood from PE patients are remarkably increased compared with those of normal non-pregnancies or normal pregnancies, while the percentage of CD3+/CD62L+ cells is significantly reduced in PE patients compared to normal pregnancies. Furthermore, CD62L levels in granulocytes of periphery blood of PE patients are significantly higher than non-pregnancies, but significantly lower than normal pregnancies. To characterize the effects of intracellular reactive oxygen species (ROS) on T lymphocyte activation in PE patients, PBMCs from normal pregnancies were challenged with H2O2, and intracellular ROS levels in neutrophil granulocytes, as well as T cell surface marker levels, have been determined. We confirm that H2O2 exposure increases intracellular ROS levels in neutrophil granulocytes, and increases the proportion of CD3+/HLA-DR+ cells, but does not alter the percentage of CD3+/CD62L+ cells in PBMCs. Our study has confirmed dysregulated CD3+/HLA-DR+ and CD3+/CD62L+ T lymphocytes in PE patient peripheral blood, and the dysregulative effects of H2O2 on T lymphocyte activation, suggesting a novel mechanism of immune activation in PE.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalInnate Immunity
Volume24
Issue number4
DOIs
StatePublished - May 1 2018

Fingerprint

Pre-Eclampsia
Hydrogen Peroxide
T-Lymphocytes
HLA-DR Antigens
Pregnancy
Granulocytes
Reactive Oxygen Species
Lymphocyte Activation
Neutrophils
Neutrophil Activation
Blood Cells
Oxidative Stress
Mortality

Keywords

  • CD62L
  • HLA-DR
  • hydrogen peroxide
  • neutrophil
  • preeclampsia
  • T lymphocyte

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

Cite this

Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells. / Lv, Jingzhu; Zhang, Xiaojie; Wang, Caizhi; Wang, Hongtao; Wang, Ting; Qian, Zhongqing.

In: Innate Immunity, Vol. 24, No. 4, 01.05.2018, p. 203-209.

Research output: Contribution to journalArticle

Lv, Jingzhu ; Zhang, Xiaojie ; Wang, Caizhi ; Wang, Hongtao ; Wang, Ting ; Qian, Zhongqing. / Hydrogen peroxide promotes the activation of preeclampsia peripheral T cells. In: Innate Immunity. 2018 ; Vol. 24, No. 4. pp. 203-209.
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