Melphalan (30 μg/ml) is completely hydrolyzed in water at 37° after 8 hr. At lower temperatures, hydrolysis proceeds at slower rates. The presence of bovine serum albumin retards hydrolysis of melphalan (30 μg/ml) in water. The melphalan hydrolysis rate is directly related to the bovine serum albumin concentration. At 37° 8 g of bovine serum albumin/100 ml of water gives a recovery rate of melphalan similar to that of human plasma. In vitro alkylation of melphalan at 37° with human plasma containing 30 μg/ml, calculated by equilibrium dialysis, methanol extraction, and high‐pressure liquid chromatographic analysis, is 30% after 8 hr.
- Antineoplastic agents—melphalan, hydrolysis, effect of bovine serum albumin, various temperatures
- Hydrolysis—melphalan, effect of bovine serum albumin, various temperatures
- Melphalan—hydrolysis, effect of bovine serum albumin, various temperatures
ASJC Scopus subject areas
- Pharmaceutical Science