Hydrophobic chalcogenide fibers for cell-based bio-optical sensors

Pierre Lucas, Mark R. Riley, Michelle A. Solis, Christophe Juncker, Jayne Collier, Dianne E. Boesewetter

Research output: Contribution to journalConference article

4 Scopus citations

Abstract

Chalcogenide fibers are shown to exhibit a hydrophobic surface behavior which results in detection enhancement for organic species in aqueous solutions. We use these fibers to monitor the infrared signature of human lung cells and detect the presence of toxic agents in the cell surrounding media. The signal is collected using a fiber evanescent wave spectroscopy set up with live human cells acting as a sensitizer for detection of minute quantities of toxicant. A monolayer of human alveolar epithelial cells form strong attachment at the surface of the fiber sensing zone and live in contact with the fiber while their IR spectra is collected remotely. Biochemical change in the living cells are detected during exposure to toxic agents. Variations in the spectroscopic features of the cells are observed in different spectral regions. Finally, the toxicity of Te2As3Se5 fibers is investigated.

Original languageEnglish (US)
Article number28
Pages (from-to)104-114
Number of pages11
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5691
DOIs
StatePublished - Jul 21 2005
EventOptical Fibers and Sensors for Medical Applications V - San Jose, CA, United States
Duration: Jan 22 2005Jan 25 2005

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Keywords

  • Bio-sensor
  • Chalcogenide glass fiber
  • Evanescent wave
  • Human lung cell
  • Hydrophobicity
  • IR spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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