Hypoxia and halothane metabolism in vivo

Release of inorganic fluoride and halothane metabolite binding to cellular constituents

L. A. Widger, A Jay Gandolfi, R. A. Van Dyke

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Fluoride release and covalent binding of halothane metabolites were studied in rats pretreated with phenobarbital and anesthestized with halothane in the presence of high (40 per cent) and low (7 per cent) oxygen tensions. The purpose of producing hypoxia was to promote the reductive pathways involved in the metabolism of halothane. Halothane anesthesia under hypoxic conditions caused a significant elevation in the plasma fluoride concentration. There was also a greater than threefold increase in covalent binding of 14C halothane metabolites to microsomal lipids in hypoxic rats. The lipid/protein binding ratio in control animals averaged 0.76, while hypoxic animals had a binding ratio of 3.24. The findings demonstrate that defluorination of halothane does occur during hypoxic conditions. It is hypothesized that the products produced by this reductive metabolic pathway are also potentially more hepatotoxic than the oxidative metabolites, based upon the increased covalent binding of halothane metabolites under hypoxic conditions.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalAnesthesiology
Volume44
Issue number3
StatePublished - 1976
Externally publishedYes

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Halothane
Fluorides
Lipids
Phenobarbital
Metabolic Networks and Pathways
Hypoxia
Protein Binding
Anesthesia
Oxygen

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Hypoxia and halothane metabolism in vivo : Release of inorganic fluoride and halothane metabolite binding to cellular constituents. / Widger, L. A.; Gandolfi, A Jay; Van Dyke, R. A.

In: Anesthesiology, Vol. 44, No. 3, 1976, p. 197-201.

Research output: Contribution to journalArticle

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