Abstract
Activity of voltage-gated K+ channels (K(V)) in pulmonary arterial smooth muscle cells (PASMC) is pivotal in controlling membrane potential, cytoplasmic free Ca2+ concentration ([Ca2+)(cyt)), and pulmonary vasomotor tone. Acute hypoxia selectively inhibits K(V) channels, depolarizes PASMC, raises [Ca2+](cyt), and causes pulmonary vasoconstriction and vascular remodeling. Prolonged hypoxia (24-60 h) decreased significantly the mRNA levels of K(V) channel α subunits, K(V)1.2 and K(V)1.5. Consistently, the protein levels of K(V)1.2 and K(V)1.5 were also decreased significantly by hypoxia (48-72 h). Nevertheless, hypoxia affected negligibly the mRNA levels of K(V) channel β subunits (K(V)β1, K(V)β2, and K(V)β3). The native K+ channels are composed of pore-forming α and auxiliary β subunits. Assembly of K(V) β subunits with α subunits confers rapid inactivation on the slowly or non-inactivating delayed rectifier K(V) channels. K(V) β subunits also function as an open-channel blocker of K(V) channels. Thus, the diminished transcription and expression of K(V) a subunits may reduce the number of K(V) channels and decrease K(V) currents. Unchanged transcription of K(V) β subunits may increase the fraction of the K(V) channel α subunits that are associated with β subunits and further reduce the total K(V) currents. These data demonstrate a novel mechanism by which chronic hypoxia may cause pulmonary vasoconstriction and hypertension.
Original language | English (US) |
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Pages (from-to) | 2347-2353 |
Number of pages | 7 |
Journal | Journal of Clinical Investigation |
Volume | 100 |
Issue number | 9 |
DOIs | |
State | Published - Nov 1 1997 |
Externally published | Yes |
Keywords
- K(V)1.2
- K(V)1.5
- Reverse transcription- PCR
- Western blotting
- α subunits
- β subunits
ASJC Scopus subject areas
- Medicine(all)