Hypoxia/aglycemia alters expression of occludin and actin in brain endothelial cells

Rachel C. Brown, Thomas P. Davis

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

The blood-brain barrier (BBB) serves as a critical organ in the maintenance of central nervous system homeostasis and is disrupted in a number of neurological disorders, including stroke. We examined the effects of hypoxia/aglycemia on the expression and localization of tight junction proteins, and on the function of the BBB in an in vitro model system. A receptor-operated/store-operated calcium channel blocker, SKF 96365, was used to determine if calcium flux was important in mediating hypoxia/aglycemia effects on the BBB. Expression of the tight junction protein occludin increased after hypoxic/aglycemic stress when cells were exposed to SKF 96365; this was correlated with partial protection of membrane localization of occludin and inhibition of the hypoxia-induced increase in permeability. Actin expression was dramatically reduced by hypoxia/aglycemia. Treatment with SKF 96365 during hypoxic stress protected monolayer permeability of sucrose, but transendothelial electrical resistances decreased with exposure to hypoxic stress regardless of treatment. Therefore, the presence of occludin at the membrane is dependent in part on calcium-sensitive signaling cascades; this provides a target for therapeutic intervention to minimize BBB disruption after stroke.

Original languageEnglish (US)
Pages (from-to)1114-1123
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number4
DOIs
StatePublished - Feb 25 2005

Keywords

  • Actin
  • Blood-brain barrier
  • Hypoxic stress
  • Occludin
  • SKF 96365
  • Tight junction
  • ZO-1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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