I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials

Thomas P Davis, Hans Schoemaker, Andrew Chen, Henry I. Yamamura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Precise and quantitative reversed-phase high performance liquid chromatographic (HPLC) procedures are described which can be used in biogenic amine and neuropeptide research. The amine procedure was applied to various pharmacological matrices including plasma, heart tissue and brain. The use of peptide HPLC as an analytical tool for various neuropeptides is illustrated by studies on des-tyrosine-γ-endorphin (DTγE) metabolism in the brain and the stability of an ACTH (ORG-2766) analogue during a chronic infusion in rats. The power of HPLC as a research tool in peptide pharmacology is described, discussed and demonstrated as an aid in the understanding of the pharmacological effects of exogenous peptides and the function of the brain.

Original languageEnglish (US)
Pages (from-to)971-987
Number of pages17
JournalLife Sciences
Volume30
Issue number12
DOIs
StatePublished - Mar 22 1982

Fingerprint

High performance liquid chromatography
Biological materials
Amines
Brain
High Pressure Liquid Chromatography
Pharmacology
Neuropeptides
Peptides
Liquids
Endorphins
Biogenic Amines
Research
Metabolism
Adrenocorticotropic Hormone
Tyrosine
Rats
Tissue
Plasmas

ASJC Scopus subject areas

  • Pharmacology

Cite this

I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials. / Davis, Thomas P; Schoemaker, Hans; Chen, Andrew; Yamamura, Henry I.

In: Life Sciences, Vol. 30, No. 12, 22.03.1982, p. 971-987.

Research output: Contribution to journalArticle

Davis, Thomas P ; Schoemaker, Hans ; Chen, Andrew ; Yamamura, Henry I. / I. High performance liquid chromatography of pharmacologically active amines and peptides in biological materials. In: Life Sciences. 1982 ; Vol. 30, No. 12. pp. 971-987.
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