Identical β cell-specific CD8+ T cell clonotypes typically reside in both peripheral blood lymphocyte and pancreatic islets

Carmen P. Wong, Rosemary Stevens, Brian Long, Li Li, Yaming Wang, Mark A. Wallet, Kevin S. Goudy, Jeffrey A. Frelinger, Roland Tisch

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A major issue regarding T cell responses in autoimmunity is how the repertoire compares between the periphery and target organ. In type 1 diabetes, the status of at-risk or diabetic individuals can be monitored by measuring β cell-specific T cells isolated from PBL, but whether these T cells accurately reflect the repertoire residing in the pancreatic islets is unclear. The TCR repertoire of disease-relevant, tetramer-sorted CD8+ T cells was examined at the single-cell level in PBL, pancreatic lymph nodes (PLN), and the islets of individual NOD mice. CDR3α and CBR3β sequences demonstrated that the same repertoire of T cells in PBL was detected in the islets and PLN, although the frequency of specific clonotypes varied. Albeit infrequent, clonotypes that were prevalent in the islets but not found in PBL were also detected. β cell Ag immunization expanded immunodominant PBL clonotypes present in the islets and PLN. These results show that insight into repertoire profiles of islet-infiltrating T cells can be obtained from PBL.

Original languageEnglish (US)
Pages (from-to)1388-1395
Number of pages8
JournalJournal of Immunology
Volume178
Issue number3
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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