Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes

Jun Isoe, Lauren E. Koch, Yurika E. Isoe, Alberto A. Rascón, Heidi E Brown, Brooke B. Massani, Roger Miesfeld

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mosquito-borne diseases are responsible for several million human deaths annually around the world. One approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. To this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. We performed bioinformatic analyses to identify putative protein-coding sequences that are specific to mosquito genomes. Systematic RNA interference (RNAi) screening of 40 mosquito-specific genes was performed by injecting double-stranded RNA (dsRNA) into female Aedes aegypti mosquitoes. This experimental approach led to the identification of eggshell organizing factor 1 (EOF1, AAEL012336), which plays an essential role in the formation and melanization of the eggshell. Eggs deposited by EOF1-deficient mosquitoes have nonmelanized fragile eggshells, and all embryos are nonviable. Scanning electron microscopy (SEM) analysis identified that exochorionic eggshell structures are strongly affected in EOF1-deficient mosquitoes. EOF1 is a potential novel target, to our knowledge, for exploring the identification and development of mosquito-selective and biosafe small-molecule inhibitors.

Original languageEnglish (US)
Pages (from-to)e3000068
JournalPLoS Biology
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Egg Shell
Aedes
egg shell
Aedes aegypti
Culicidae
Genes
Double-Stranded RNA
Bioelectric potentials
Bioinformatics
Screening
Proteins
RNA
Scanning electron microscopy
Molecules
Eggs
mosquito-borne diseases
melanization
vector control
double-stranded RNA
RNA interference

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes. / Isoe, Jun; Koch, Lauren E.; Isoe, Yurika E.; Rascón, Alberto A.; Brown, Heidi E; Massani, Brooke B.; Miesfeld, Roger.

In: PLoS Biology, Vol. 17, No. 1, 01.01.2019, p. e3000068.

Research output: Contribution to journalArticle

Isoe, Jun ; Koch, Lauren E. ; Isoe, Yurika E. ; Rascón, Alberto A. ; Brown, Heidi E ; Massani, Brooke B. ; Miesfeld, Roger. / Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes. In: PLoS Biology. 2019 ; Vol. 17, No. 1. pp. e3000068.
@article{1cc8407fa20544bdb8de72e3222066fe,
title = "Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes",
abstract = "Mosquito-borne diseases are responsible for several million human deaths annually around the world. One approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. To this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. We performed bioinformatic analyses to identify putative protein-coding sequences that are specific to mosquito genomes. Systematic RNA interference (RNAi) screening of 40 mosquito-specific genes was performed by injecting double-stranded RNA (dsRNA) into female Aedes aegypti mosquitoes. This experimental approach led to the identification of eggshell organizing factor 1 (EOF1, AAEL012336), which plays an essential role in the formation and melanization of the eggshell. Eggs deposited by EOF1-deficient mosquitoes have nonmelanized fragile eggshells, and all embryos are nonviable. Scanning electron microscopy (SEM) analysis identified that exochorionic eggshell structures are strongly affected in EOF1-deficient mosquitoes. EOF1 is a potential novel target, to our knowledge, for exploring the identification and development of mosquito-selective and biosafe small-molecule inhibitors.",
author = "Jun Isoe and Koch, {Lauren E.} and Isoe, {Yurika E.} and Rasc{\'o}n, {Alberto A.} and Brown, {Heidi E} and Massani, {Brooke B.} and Roger Miesfeld",
year = "2019",
month = "1",
day = "1",
doi = "10.1371/journal.pbio.3000068",
language = "English (US)",
volume = "17",
pages = "e3000068",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes

AU - Isoe, Jun

AU - Koch, Lauren E.

AU - Isoe, Yurika E.

AU - Rascón, Alberto A.

AU - Brown, Heidi E

AU - Massani, Brooke B.

AU - Miesfeld, Roger

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Mosquito-borne diseases are responsible for several million human deaths annually around the world. One approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. To this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. We performed bioinformatic analyses to identify putative protein-coding sequences that are specific to mosquito genomes. Systematic RNA interference (RNAi) screening of 40 mosquito-specific genes was performed by injecting double-stranded RNA (dsRNA) into female Aedes aegypti mosquitoes. This experimental approach led to the identification of eggshell organizing factor 1 (EOF1, AAEL012336), which plays an essential role in the formation and melanization of the eggshell. Eggs deposited by EOF1-deficient mosquitoes have nonmelanized fragile eggshells, and all embryos are nonviable. Scanning electron microscopy (SEM) analysis identified that exochorionic eggshell structures are strongly affected in EOF1-deficient mosquitoes. EOF1 is a potential novel target, to our knowledge, for exploring the identification and development of mosquito-selective and biosafe small-molecule inhibitors.

AB - Mosquito-borne diseases are responsible for several million human deaths annually around the world. One approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. To this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. We performed bioinformatic analyses to identify putative protein-coding sequences that are specific to mosquito genomes. Systematic RNA interference (RNAi) screening of 40 mosquito-specific genes was performed by injecting double-stranded RNA (dsRNA) into female Aedes aegypti mosquitoes. This experimental approach led to the identification of eggshell organizing factor 1 (EOF1, AAEL012336), which plays an essential role in the formation and melanization of the eggshell. Eggs deposited by EOF1-deficient mosquitoes have nonmelanized fragile eggshells, and all embryos are nonviable. Scanning electron microscopy (SEM) analysis identified that exochorionic eggshell structures are strongly affected in EOF1-deficient mosquitoes. EOF1 is a potential novel target, to our knowledge, for exploring the identification and development of mosquito-selective and biosafe small-molecule inhibitors.

UR - http://www.scopus.com/inward/record.url?scp=85059795759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059795759&partnerID=8YFLogxK

U2 - 10.1371/journal.pbio.3000068

DO - 10.1371/journal.pbio.3000068

M3 - Article

C2 - 30620728

AN - SCOPUS:85059795759

VL - 17

SP - e3000068

JO - PLoS Biology

JF - PLoS Biology

SN - 1544-9173

IS - 1

ER -