Identification and characterization of a rhizosphere β-galactosidase from Pisum sativum L.

F. Wen, R. Celoy, I. Price, J. J. Ebolo, Martha C Hawes

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Plant enzyme activities in the rhizosphere potentially are a resource for improved plant nutrition, soil fertility, bioremediation, and disease resistance. Here we report that a border cell specific β-galactosidase is secreted into the acidic extracellular environment surrounding root tips of pea, as well as bean, alfalfa, barrel medic, sorghum, and maize. No enzyme activity was detected in radish and Arabidopsis, species that do not produce viable border cells. The secreted enzyme activity was inhibited by galactose and 2-phenylethyl 1-thio-β-d-galactopyranoside (PETG) at concentrations that altered root growth without causing cell death. A tomato galactanase encoding gene was used as a probe to isolate a full length pea cDNA clone (BRDgal1) from a root cap-border cell cDNA library. Southern blot analysis using full length BRDgal1 as a probe revealed 1-2 related sequences within the pea genome. BRDgal1 mRNA expression was analysed by whole mount in situ hybridization (WISH) and found to occur in the outermost peripheral layer of the cap and in suspensions of detached border cells. No expression was detected within the body of the root cap. Repeated efforts to develop viable hairy root clones expressing BRDgal1 antisense mRNA under the control of the CaMV35S promoter, whose expression in the root cap is limited to cells at the root cap periphery only during root emergence, were unsuccessful. These data suggest that altered expression of this enzyme is deleterious to early root development.

Original languageEnglish (US)
Pages (from-to)133-144
Number of pages12
JournalPlant and Soil
Volume304
Issue number1-2
DOIs
StatePublished - Mar 2008

Fingerprint

galactosidases
root cap
Pisum sativum
rhizosphere
peas
enzyme activity
cells
clones
Medicago truncatula
bioremediation
radishes
plant nutrition
root tips
cDNA libraries
clone
galactose
Southern blotting
in situ hybridization
disease resistance
soil fertility

Keywords

  • Rhizosphere
  • Root cap
  • Root cap secretome
  • Root exudates

ASJC Scopus subject areas

  • Soil Science
  • Agronomy and Crop Science
  • Plant Science

Cite this

Identification and characterization of a rhizosphere β-galactosidase from Pisum sativum L. / Wen, F.; Celoy, R.; Price, I.; Ebolo, J. J.; Hawes, Martha C.

In: Plant and Soil, Vol. 304, No. 1-2, 03.2008, p. 133-144.

Research output: Contribution to journalArticle

Wen, F. ; Celoy, R. ; Price, I. ; Ebolo, J. J. ; Hawes, Martha C. / Identification and characterization of a rhizosphere β-galactosidase from Pisum sativum L. In: Plant and Soil. 2008 ; Vol. 304, No. 1-2. pp. 133-144.
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