Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

Shao Hua Gu, Jing Jiang Zhou, Shang Gao, Da Hai Wang, Xianchun Li, Yu Yuan Guo, Yong Jun Zhang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

Original languageEnglish (US)
Article number13800
JournalScientific Reports
Volume5
DOIs
StatePublished - Sep 8 2015

Fingerprint

Spodoptera
Tobacco
Genes
Lepidoptera
Insects
Insect Genes
odorant-binding protein
Smell
Transcriptome

ASJC Scopus subject areas

  • General

Cite this

Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura. / Gu, Shao Hua; Zhou, Jing Jiang; Gao, Shang; Wang, Da Hai; Li, Xianchun; Guo, Yu Yuan; Zhang, Yong Jun.

In: Scientific Reports, Vol. 5, 13800, 08.09.2015.

Research output: Contribution to journalArticle

Gu, Shao Hua ; Zhou, Jing Jiang ; Gao, Shang ; Wang, Da Hai ; Li, Xianchun ; Guo, Yu Yuan ; Zhang, Yong Jun. / Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura. In: Scientific Reports. 2015 ; Vol. 5.
@article{30a3292ebe6044e495a62ee9d21af200,
title = "Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura",
abstract = "Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.",
author = "Gu, {Shao Hua} and Zhou, {Jing Jiang} and Shang Gao and Wang, {Da Hai} and Xianchun Li and Guo, {Yu Yuan} and Zhang, {Yong Jun}",
year = "2015",
month = "9",
day = "8",
doi = "10.1038/srep13800",
language = "English (US)",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Identification and comparative expression analysis of odorant binding protein genes in the tobacco cutworm Spodoptera litura

AU - Gu, Shao Hua

AU - Zhou, Jing Jiang

AU - Gao, Shang

AU - Wang, Da Hai

AU - Li, Xianchun

AU - Guo, Yu Yuan

AU - Zhang, Yong Jun

PY - 2015/9/8

Y1 - 2015/9/8

N2 - Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

AB - Insect odorant binding proteins (OBPs) are thought to involve in insects' olfaction perception. In the present study, we identified 38 OBP genes from the antennal transcriptomes of Spodoptera litura. Tissue expression profiles analysis revealed that 17 of the 38 SlitOBP transcripts were uniquely or primarily expressed in the antennae of both sexes, suggesting their putative role in chemoreception. The RPKM value analysis revealed that seven OBPs (SlitPBP1-3, SlitGOBP1-2, SlitOBP3 and SlitOBP5) are highly abundant in male and female antennae. Most S. litura antennal unigenes had high homology with Lepidoptera insects, especially genes of the genus Spodoptera. Phylogenetic analysis of the Lepidoptera OBPs demonstrated that the OBP genes from the genus Spodoptera (S. litura, Spodoptera littoralis and Spodoptera exigua) had a relatively close evolutionary relationship. Some regular patterns and key conserved motifs of OBPs in genus Spodoptera are identified by MEME, and their putative roles in detecting odorants are discussed here. The motif-patterns between Lepidoptera OBPs and CSPs are also compared. The SlitOBPs identified here provide a starting point to facilitate functional studies of insect OBPs at the molecular level both in vivo and in vitro.

UR - http://www.scopus.com/inward/record.url?scp=84941093087&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941093087&partnerID=8YFLogxK

U2 - 10.1038/srep13800

DO - 10.1038/srep13800

M3 - Article

C2 - 26346731

AN - SCOPUS:84941093087

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 13800

ER -