Identification and genetic characterization of yeasts isolated from freshwater prawns, Macrobrachium rosenbergii de Man, in Taiwan

C. C. Lu, Feng-Jyu Tang-Nelson, S. N. Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Since 1987, farmers in southern Taiwan have reported significant disease-related mortalities-in freshwater prawns. Most mortalities have occurred during the winter, and usually almost all the adult prawns die within 4-5 days after clinical signs appear. Histopathological studies show that cells in hepatopancreatic ducts and tubules are vacuolized and degenerated. Large numbers of membrane-bound yeast aggregates are observed in the affected tissues. Two hundred and seventeen yeast isolates were obtained from diseased prawns, pond water and sediments from six areas. DNA restriction fragment length polymorphism (RFLP) was used to analyse the yeast genomes and to categorize them into five groups. Conventional biochemical and metabolic methods were then used to identify the yeasts of each group. The results showed that two strains of Candida sake (I and II), Pichia anomala, Endomyces fibuliger and Candida famata were present. In addition, the RAPD (random amplified polymorphic DNA) method was used to determine their genomic similarities. Two strains (I and II) of C. sake were the most similar (72%). C. sake I appears to be the primary causative agent of disease, based on frequencies of occurrence of the yeasts found in the diseased prawns.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalJournal of Fish Diseases
Volume21
Issue number3
DOIs
StatePublished - May 1 1998
Externally publishedYes

Fingerprint

Palaemonidae
Macrobrachium
Macrobrachium rosenbergii
Fresh Water
Taiwan
yeast
Yeasts
yeasts
shrimp
Candida
Endomyces
Candida saitoana
Wickerhamomyces anomalus
DNA
mortality
random amplified polymorphic DNA technique
Pichia
Mortality
cell aggregates
Restriction Fragment Length Polymorphisms

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary (miscalleneous)

Cite this

Identification and genetic characterization of yeasts isolated from freshwater prawns, Macrobrachium rosenbergii de Man, in Taiwan. / Lu, C. C.; Tang-Nelson, Feng-Jyu; Chen, S. N.

In: Journal of Fish Diseases, Vol. 21, No. 3, 01.05.1998, p. 185-192.

Research output: Contribution to journalArticle

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