Identification and partial purification of a lectin on the surface of the sporozoite of Cryptosporidium parvum

D. M. Thea, M. E A Pereira, D. Kotler, Charles R Sterling, G. T. Keusch

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A human-derived isolate of Cryptosporidium parvum from a symptomatic patient with the acquired immunodeficiency syndrome was expanded in vivo by infecting a neonatal calf with 108 oocysts. Sporozoites were isolated from 4 x 1010 oocysts harvested from this single infection, and the characteristics of mixed hemagglutination (HA) with rabbit erythrocytes were determined. Sporozoite HA was inhibited by bovine submaxillary mucin (BSM), hog gastric mucin, and orosomucoid, but not by simple sugars, including sialic acid. Carbohydrate-inhibitable HA (lectin) activity increased with sporozoite lysis and was associated with the sporozoite membrane fractions. The ability of intact sporozoites to form rosettes around erythrocytes indicates that the HA (lectin) is, at least in part, present on the parasite surface. Hemagglutination (lectin) activity was partially purified from sporozoite lysates by affinity chromatography with BSM coupled to Sepharose-4B. Best elution was obtained with ethylene glycol and NaCl, which resulted in enrichment of 6 bands compared to the crude starting lysate (Mr = 60, 24, 22, 20, and 15 kDa and a 40-kDa doublet). Our results indicate that an HA (lectin) activity is present on the surface of intact sporozoites where it could play a role in cell-to-cell interactions with eukaryotic targets.

Original languageEnglish (US)
Pages (from-to)886-893
Number of pages8
JournalJournal of Parasitology
Volume78
Issue number5
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Sporozoites
sporozoites
Lectins
lectins
Hemagglutination
hemagglutination
purification
lysis
mucins
ethylene
chromatography
carbohydrate
sugar
parasite
membrane
Oocysts
acid
oocysts
erythrocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Identification and partial purification of a lectin on the surface of the sporozoite of Cryptosporidium parvum. / Thea, D. M.; Pereira, M. E A; Kotler, D.; Sterling, Charles R; Keusch, G. T.

In: Journal of Parasitology, Vol. 78, No. 5, 1992, p. 886-893.

Research output: Contribution to journalArticle

Thea, D. M. ; Pereira, M. E A ; Kotler, D. ; Sterling, Charles R ; Keusch, G. T. / Identification and partial purification of a lectin on the surface of the sporozoite of Cryptosporidium parvum. In: Journal of Parasitology. 1992 ; Vol. 78, No. 5. pp. 886-893.
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