Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer

Robert S. Goodenow, Minnie Mcmillan, Anders Örn, Margery Nicolson, Norman Davidson, Jeffrey A Frelinger, Leroy Hood

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2Ld gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing Ld molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign Ld gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen Ld molecules by two-dimensional electrophoretic analysis of H-2Ld immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2Ld gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.

Original languageEnglish (US)
Pages (from-to)677-679
Number of pages3
JournalScience
Volume215
Issue number4533
StatePublished - 1982
Externally publishedYes

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DNA
Genes
Clone Cells
Thymidine Kinase
Inbred C3H Mouse
Hybridomas
Simplexvirus
Multigene Family
Spleen
Monoclonal Antibodies
Antigens

ASJC Scopus subject areas

  • General

Cite this

Goodenow, R. S., Mcmillan, M., Örn, A., Nicolson, M., Davidson, N., Frelinger, J. A., & Hood, L. (1982). Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. Science, 215(4533), 677-679.

Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. / Goodenow, Robert S.; Mcmillan, Minnie; Örn, Anders; Nicolson, Margery; Davidson, Norman; Frelinger, Jeffrey A; Hood, Leroy.

In: Science, Vol. 215, No. 4533, 1982, p. 677-679.

Research output: Contribution to journalArticle

Goodenow, RS, Mcmillan, M, Örn, A, Nicolson, M, Davidson, N, Frelinger, JA & Hood, L 1982, 'Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer', Science, vol. 215, no. 4533, pp. 677-679.
Goodenow RS, Mcmillan M, Örn A, Nicolson M, Davidson N, Frelinger JA et al. Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. Science. 1982;215(4533):677-679.
Goodenow, Robert S. ; Mcmillan, Minnie ; Örn, Anders ; Nicolson, Margery ; Davidson, Norman ; Frelinger, Jeffrey A ; Hood, Leroy. / Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer. In: Science. 1982 ; Vol. 215, No. 4533. pp. 677-679.
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