Abstract
Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+ cells). The Ck6a+ cells are present within a specialized niche in the basal cell compartment in fetal, juvenile and adult prostate tissue, and within the stem cell-enriched urogenital sinus. In adult normal prostate tissue, the average abundance of Ck6a+ cells was 4.9%. With proliferative stimuli in the prostate organ culture model, in which the epithelial-stromal interaction was maintained, a remarkable increase of Ck 6a expression was noticed to up to 64.9%. The difference in cytokeratin 6a expression between the normal adult prostate and the prostate organ culture model was statistically significant (p < 0.0001). Within the prostate organ culture model the increase of cytokeratin 6a-expressing cells significantly correlated with increased proliferation index (r = 0.7616, p = 0.0467) The Ck6a+ cells were capable of differentiation as indicated by their expression of luminal cell markers such as ZO-1 and prostate specific antigen (PSA). Our data indicate that Ck6a+ cells represent a prostatic epithelial stem cell candidate possessing high potential for proliferation and differentiation. Since the development of benign prostatic hyperplasia and prostate carcinogenesis are disorders of proliferation and differentiation, the Ck6a+ cells may represent a major element in the development of these diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 341-354 |
| Number of pages | 14 |
| Journal | European Journal of Cell Biology |
| Volume | 84 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Mar 9 2005 |
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Keywords
- Cytokeratin 6
- Glandular epithelium
- Prostate
- Stem cells
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
Identification of a stem cell candidate in the normal human prostate gland. / Schmelz, Monika; Moll, Roland; Hesse, Ulrike; Rama Rao, Anil P; Gandolfi, A Jay; Hasan, Shirin R.; Bartholdi, Marty; Cress, Anne E.
In: European Journal of Cell Biology, Vol. 84, No. 2-3, 09.03.2005, p. 341-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a stem cell candidate in the normal human prostate gland
AU - Schmelz, Monika
AU - Moll, Roland
AU - Hesse, Ulrike
AU - Rama Rao, Anil P
AU - Gandolfi, A Jay
AU - Hasan, Shirin R.
AU - Bartholdi, Marty
AU - Cress, Anne E
PY - 2005/3/9
Y1 - 2005/3/9
N2 - Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+ cells). The Ck6a+ cells are present within a specialized niche in the basal cell compartment in fetal, juvenile and adult prostate tissue, and within the stem cell-enriched urogenital sinus. In adult normal prostate tissue, the average abundance of Ck6a+ cells was 4.9%. With proliferative stimuli in the prostate organ culture model, in which the epithelial-stromal interaction was maintained, a remarkable increase of Ck 6a expression was noticed to up to 64.9%. The difference in cytokeratin 6a expression between the normal adult prostate and the prostate organ culture model was statistically significant (p < 0.0001). Within the prostate organ culture model the increase of cytokeratin 6a-expressing cells significantly correlated with increased proliferation index (r = 0.7616, p = 0.0467) The Ck6a+ cells were capable of differentiation as indicated by their expression of luminal cell markers such as ZO-1 and prostate specific antigen (PSA). Our data indicate that Ck6a+ cells represent a prostatic epithelial stem cell candidate possessing high potential for proliferation and differentiation. Since the development of benign prostatic hyperplasia and prostate carcinogenesis are disorders of proliferation and differentiation, the Ck6a+ cells may represent a major element in the development of these diseases.
AB - Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+ cells). The Ck6a+ cells are present within a specialized niche in the basal cell compartment in fetal, juvenile and adult prostate tissue, and within the stem cell-enriched urogenital sinus. In adult normal prostate tissue, the average abundance of Ck6a+ cells was 4.9%. With proliferative stimuli in the prostate organ culture model, in which the epithelial-stromal interaction was maintained, a remarkable increase of Ck 6a expression was noticed to up to 64.9%. The difference in cytokeratin 6a expression between the normal adult prostate and the prostate organ culture model was statistically significant (p < 0.0001). Within the prostate organ culture model the increase of cytokeratin 6a-expressing cells significantly correlated with increased proliferation index (r = 0.7616, p = 0.0467) The Ck6a+ cells were capable of differentiation as indicated by their expression of luminal cell markers such as ZO-1 and prostate specific antigen (PSA). Our data indicate that Ck6a+ cells represent a prostatic epithelial stem cell candidate possessing high potential for proliferation and differentiation. Since the development of benign prostatic hyperplasia and prostate carcinogenesis are disorders of proliferation and differentiation, the Ck6a+ cells may represent a major element in the development of these diseases.
KW - Cytokeratin 6
KW - Glandular epithelium
KW - Prostate
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=14844365502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14844365502&partnerID=8YFLogxK
U2 - 10.1016/j.ejcb.2004.12.019
DO - 10.1016/j.ejcb.2004.12.019
M3 - Article
C2 - 15819412
AN - SCOPUS:14844365502
VL - 84
SP - 341
EP - 354
JO - European Journal of Cell Biology
JF - European Journal of Cell Biology
SN - 0171-9335
IS - 2-3
ER -