Identification of a stem cell candidate in the normal human prostate gland

Monika Schmelz, Roland Moll, Ulrike Hesse, Anil P Rama Rao, A Jay Gandolfi, Shirin R. Hasan, Marty Bartholdi, Anne E Cress

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Stem cells of the human prostate gland have not yet been identified utilizing a structural biomarker. We have discovered a new prostatic epithelial cell phenotype-expressing cytokeratin 6a (Ck6a+ cells). The Ck6a+ cells are present within a specialized niche in the basal cell compartment in fetal, juvenile and adult prostate tissue, and within the stem cell-enriched urogenital sinus. In adult normal prostate tissue, the average abundance of Ck6a+ cells was 4.9%. With proliferative stimuli in the prostate organ culture model, in which the epithelial-stromal interaction was maintained, a remarkable increase of Ck 6a expression was noticed to up to 64.9%. The difference in cytokeratin 6a expression between the normal adult prostate and the prostate organ culture model was statistically significant (p < 0.0001). Within the prostate organ culture model the increase of cytokeratin 6a-expressing cells significantly correlated with increased proliferation index (r = 0.7616, p = 0.0467) The Ck6a+ cells were capable of differentiation as indicated by their expression of luminal cell markers such as ZO-1 and prostate specific antigen (PSA). Our data indicate that Ck6a+ cells represent a prostatic epithelial stem cell candidate possessing high potential for proliferation and differentiation. Since the development of benign prostatic hyperplasia and prostate carcinogenesis are disorders of proliferation and differentiation, the Ck6a+ cells may represent a major element in the development of these diseases.

Original languageEnglish (US)
Pages (from-to)341-354
Number of pages14
JournalEuropean Journal of Cell Biology
Volume84
Issue number2-3
DOIs
StatePublished - Mar 9 2005

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Prostate
Stem Cells
Keratin-6
Organ Culture Techniques
Epithelial Cells
Prostatic Hyperplasia
Prostate-Specific Antigen
Cell Differentiation
Carcinogenesis
Biomarkers
Phenotype

Keywords

  • Cytokeratin 6
  • Glandular epithelium
  • Prostate
  • Stem cells

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Identification of a stem cell candidate in the normal human prostate gland. / Schmelz, Monika; Moll, Roland; Hesse, Ulrike; Rama Rao, Anil P; Gandolfi, A Jay; Hasan, Shirin R.; Bartholdi, Marty; Cress, Anne E.

In: European Journal of Cell Biology, Vol. 84, No. 2-3, 09.03.2005, p. 341-354.

Research output: Contribution to journalArticle

Schmelz, Monika ; Moll, Roland ; Hesse, Ulrike ; Rama Rao, Anil P ; Gandolfi, A Jay ; Hasan, Shirin R. ; Bartholdi, Marty ; Cress, Anne E. / Identification of a stem cell candidate in the normal human prostate gland. In: European Journal of Cell Biology. 2005 ; Vol. 84, No. 2-3. pp. 341-354.
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