Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

Eva V. Varga, Dagmar Stropova, Marc Rubenzik, Man Wang, Robert S. Landsman, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.

Original languageEnglish (US)
JournalEuropean Journal of Pharmacology
Volume348
Issue number2-3
DOIs
StatePublished - May 8 1998
Externally publishedYes

Fingerprint

Cricetulus
Adenylyl Cyclases
Isoenzymes
Ovary
G-Protein-Coupled Receptors
Reverse Transcriptase Polymerase Chain Reaction
Complementary DNA
Fibroblasts
Cell Line
Messenger RNA
adenylyl cyclase 7

Keywords

  • Adenylyl cyclase isoenzyme
  • Chinese hamster ovary (CHO) cell
  • Mouse fibroblast (B82) cell

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Varga, E. V., Stropova, D., Rubenzik, M., Wang, M., Landsman, R. S., Roeske, W. R., & Yamamura, H. I. (1998). Identification of adenylyl cyclase isoenzymes in CHO and B82 cells. European Journal of Pharmacology, 348(2-3). https://doi.org/10.1016/S0014-2999(98)00258-1

Identification of adenylyl cyclase isoenzymes in CHO and B82 cells. / Varga, Eva V.; Stropova, Dagmar; Rubenzik, Marc; Wang, Man; Landsman, Robert S.; Roeske, William R.; Yamamura, Henry I.

In: European Journal of Pharmacology, Vol. 348, No. 2-3, 08.05.1998.

Research output: Contribution to journalArticle

Varga, EV, Stropova, D, Rubenzik, M, Wang, M, Landsman, RS, Roeske, WR & Yamamura, HI 1998, 'Identification of adenylyl cyclase isoenzymes in CHO and B82 cells', European Journal of Pharmacology, vol. 348, no. 2-3. https://doi.org/10.1016/S0014-2999(98)00258-1
Varga, Eva V. ; Stropova, Dagmar ; Rubenzik, Marc ; Wang, Man ; Landsman, Robert S. ; Roeske, William R. ; Yamamura, Henry I. / Identification of adenylyl cyclase isoenzymes in CHO and B82 cells. In: European Journal of Pharmacology. 1998 ; Vol. 348, No. 2-3.
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