Identification of adenylyl cyclase isoenzymes in CHO and B82 cells

Eva V. Varga, Dagmar Stropova, Marc Rubenzik, Man Wang, Robert S. Landsman, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.

Original languageEnglish (US)
Pages (from-to)R1-R2
JournalEuropean Journal of Pharmacology
Issue number2-3
StatePublished - May 8 1998
Externally publishedYes


  • Adenylyl cyclase isoenzyme
  • Chinese hamster ovary (CHO) cell
  • Mouse fibroblast (B82) cell

ASJC Scopus subject areas

  • Pharmacology

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