Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.

N. Stromberg, C. Deal, G. Nyberg, S. Normark, Magdalene "Maggie" So, K. A. Karlsson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Different strains and isogenic variants of Neisseria gonorrhoeae were assayed for their ability to bind glycolipids extracted from various sources. Among a large number of reference glycolipids, binding was observed only to lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], isoglobotriaosylceramide [Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriaosylceramide [GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer]. The latter two glycolipids bound gonococci with the highest affinity. Lactosylceramide and gangliotriaosylceramide were found in glycolipid preparations from ME180 cells, an epithelial cell line derived from a human cervical carcinoma, and thus are possible receptors for gonococci. The gonococcal surface component that bound the above glycolipids is a protein distinct from pilin and protein II.

Original languageEnglish (US)
Pages (from-to)4902-4906
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number13
StatePublished - Jul 1988
Externally publishedYes

Fingerprint

Neisseria gonorrhoeae
Glycolipids
Carbohydrates
Fimbriae Proteins
Proteins
Epithelial Cells
Carcinoma
Cell Line
glucose tetrasaccharide
CDw17 antigen

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae. / Stromberg, N.; Deal, C.; Nyberg, G.; Normark, S.; So, Magdalene "Maggie"; Karlsson, K. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 13, 07.1988, p. 4902-4906.

Research output: Contribution to journalArticle

@article{d0c11d1c1d6b4af5a72ee4e5705cfc8e,
title = "Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.",
abstract = "Different strains and isogenic variants of Neisseria gonorrhoeae were assayed for their ability to bind glycolipids extracted from various sources. Among a large number of reference glycolipids, binding was observed only to lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], isoglobotriaosylceramide [Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriaosylceramide [GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer]. The latter two glycolipids bound gonococci with the highest affinity. Lactosylceramide and gangliotriaosylceramide were found in glycolipid preparations from ME180 cells, an epithelial cell line derived from a human cervical carcinoma, and thus are possible receptors for gonococci. The gonococcal surface component that bound the above glycolipids is a protein distinct from pilin and protein II.",
author = "N. Stromberg and C. Deal and G. Nyberg and S. Normark and So, {Magdalene {"}Maggie{"}} and Karlsson, {K. A.}",
year = "1988",
month = "7",
language = "English (US)",
volume = "85",
pages = "4902--4906",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "13",

}

TY - JOUR

T1 - Identification of carbohydrate structures that are possible receptors for Neisseria gonorrhoeae.

AU - Stromberg, N.

AU - Deal, C.

AU - Nyberg, G.

AU - Normark, S.

AU - So, Magdalene "Maggie"

AU - Karlsson, K. A.

PY - 1988/7

Y1 - 1988/7

N2 - Different strains and isogenic variants of Neisseria gonorrhoeae were assayed for their ability to bind glycolipids extracted from various sources. Among a large number of reference glycolipids, binding was observed only to lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], isoglobotriaosylceramide [Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriaosylceramide [GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer]. The latter two glycolipids bound gonococci with the highest affinity. Lactosylceramide and gangliotriaosylceramide were found in glycolipid preparations from ME180 cells, an epithelial cell line derived from a human cervical carcinoma, and thus are possible receptors for gonococci. The gonococcal surface component that bound the above glycolipids is a protein distinct from pilin and protein II.

AB - Different strains and isogenic variants of Neisseria gonorrhoeae were assayed for their ability to bind glycolipids extracted from various sources. Among a large number of reference glycolipids, binding was observed only to lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], isoglobotriaosylceramide [Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriaosylceramide [GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer]. The latter two glycolipids bound gonococci with the highest affinity. Lactosylceramide and gangliotriaosylceramide were found in glycolipid preparations from ME180 cells, an epithelial cell line derived from a human cervical carcinoma, and thus are possible receptors for gonococci. The gonococcal surface component that bound the above glycolipids is a protein distinct from pilin and protein II.

UR - http://www.scopus.com/inward/record.url?scp=0024044196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024044196&partnerID=8YFLogxK

M3 - Article

VL - 85

SP - 4902

EP - 4906

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 13

ER -