Identification of differentially expressed genes in shrimp (Penaeus stylirostris) infected with White spot syndrome virus by cDNA microarrays

A. K. Dhar, A. Dettori, M. M. Roux, K. R. Klimpel, B. Read

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

White spot syndrome virus (WSSV) is currently the most important viral pathogen infecting penaeid shrimp worldwide. Although considerable progress has been made in characterizing the WSSV genome and developing detection methods, information pertaining to host genes involved in WSSV pathogenesis is limited. We examined the potential of cDNA microarray analysis to study gene expression in WSSV-infected shrimp. Shrimp cDNAs were printed as low-density arrays on glass slides and were hybridized with Cy3/Cy5 labeled probes derived from RNA isolated from healthy and WSSV-infected shrimp. Genes that code for proteins that are relevant to crustacean immunity, structural proteins, as well as proteins of unknown function were among those whose mRNA expression was altered upon WSSV infection. To validate the microarray data, the temporal expression of three differentially expressed genes, an immune gene (C-type lectin-1), a structural gene (40S ribosomal protein), and a gene involved in lipid metabolism (fatty acid binding protein) was measured in healthy and WSSV-infected shrimp by real-time RT-PCR. The data suggest that WSSV infection alters the expression of a wide array of cellular genes, and provides a framework for further studies aimed at identifying genes whose function may provide insight into the mechanism of WSSV infection in shrimp.

Original languageEnglish (US)
Pages (from-to)2381-2396
Number of pages16
JournalArchives of virology
Volume148
Issue number12
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

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White spot syndrome virus 1
Penaeidae
Oligonucleotide Array Sequence Analysis
Genes
Virus Diseases
C-Type Lectins
RNA Probes
Fatty Acid-Binding Proteins
Proteins
Ribosomal Proteins
Microarray Analysis
Lipid Metabolism
Glass
Real-Time Polymerase Chain Reaction
Immunity
Complementary DNA
Genome

ASJC Scopus subject areas

  • Virology

Cite this

Identification of differentially expressed genes in shrimp (Penaeus stylirostris) infected with White spot syndrome virus by cDNA microarrays. / Dhar, A. K.; Dettori, A.; Roux, M. M.; Klimpel, K. R.; Read, B.

In: Archives of virology, Vol. 148, No. 12, 01.12.2003, p. 2381-2396.

Research output: Contribution to journalArticle

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