Identification of dominant indigenous Rhizobium meliloti by plasmid profiles and intrinsic antibiotic resistance

M. Shishido, Ian L Pepper

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Yields of alfalfa (Medicago sativa L.) in irrigated production agriculture in the southwest U.S.A. varies widely, but is generally higher than in other areas of the U.S.A. Since nitrogen fertilizer is rarely applied, high yields are likely to be due in part to biological N2 fixation in the symbiotic association between alfalfa and Rhizobium meliloti. Dominant types of R. meliloti responsible for N2 fixation were identified from nodule isolates collected from five locations throughout the state of Arizona, which were not known to have been inoculated. The locations were sampled in the winter of 1987 and the summer of 1988. The dominant types (≥20% nodule occupancy at each sampling site) were identified through plasmid profile analysis and intrinsic antibiotic resistance patterns. Four types were found to be dominant throughout the state. A single example of each of these four dominant types and a commercial strain (Nitragin Co., Milwaukee, Wis.) were of equal symbiotic effectiveness as M. sativa cv. Lew. No significant differences were found (P ≥ 0.05) in shoot weight, root weight, nodule weight, acetylene reduction and total plant N content. Several of the selected cultures may have potential as inoculants for use in arid lands due to their effectiveness and their ability to survive in the extreme environmental conditions prevailing in the soils from which they were isolated.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalSoil Biology and Biochemistry
Volume22
Issue number1
DOIs
StatePublished - 1990

Fingerprint

Sinorhizobium meliloti
Ensifer meliloti
Medicago sativa
antibiotic resistance
Microbial Drug Resistance
rhizobacterium
plasmid
plasmids
Plasmids
Nitrogen fertilizers
Anti-Bacterial Agents
alfalfa
Acetylene
Nitrogen Fixation
fixation
Agriculture
acetylene reduction
Association reactions
acetylene
Sampling

ASJC Scopus subject areas

  • Soil Science
  • Biochemistry
  • Ecology

Cite this

Identification of dominant indigenous Rhizobium meliloti by plasmid profiles and intrinsic antibiotic resistance. / Shishido, M.; Pepper, Ian L.

In: Soil Biology and Biochemistry, Vol. 22, No. 1, 1990, p. 11-16.

Research output: Contribution to journalArticle

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