Identification of early interactions between Francisella and the host

Lydia M. Roberts, Shraddha Tuladhar, Shaun P. Steele, Kristina J. Riebe, Ching Ju Chen, R. Ian Cumming, Sarah Seay, Richard Frothingham, Gregory D. Sempowski, Thomas H. Kawula, Jeffrey A. Frelinger

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The adaptive immune response to Francisella tularensis is dependent on the route of inoculation. Intradermal inoculation with the F. tularensis live vaccine strain (LVS) results in a robust Th1 response in the lungs, whereas intranasal inoculation produces fewer Th1 cells and instead many Th17 cells. Interestingly, bacterial loads in the lungs are similar early after inoculation by these two routes. We hypothesize that the adaptive immune response is influenced by local events in the lungs, such as the type of cells that are first infected with Francisella. Using fluorescence-activated cell sorting, we identified alveolar macrophages as the first cell type infected in the lungs of mice intranasally inoculated with F. novicida U112, LVS, or F. tularensis Schu S4. Following bacterial dissemination from the skin to the lung, interstitial macrophages or neutrophils are infected. Overall, we identified the early interactions between Francisella and the host following two different routes of inoculation.

Original languageEnglish (US)
Pages (from-to)2504-2510
Number of pages7
JournalInfection and Immunity
Volume82
Issue number6
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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