Identification of fluid and substrate chemistry based on automatic pattern recognition of stains

Namwon Kim, Zhenguo Li, Cedric Hurth, Frederic Zenhausern, Shih Fu Chang, Daniel Attinger

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

This study proposes that images of stains from 100-nanolitre drops can be automatically identified as signatures of fluid composition and substrate chemistry, for e.g. rapid biological testing. Two datasets of stain images are produced and made available online, one with consumable fluids, and the other with biological fluids. Classification algorithms are used to identify an unknown stain by measuring its similarity to representative examples of predefined categories. The accuracy ranges from 80 to 94%, compared to an accuracy by random assignment of 3 to 4%. Clustering algorithms are also applied to group unknown stain images into a number of clusters each likely to correspond to similar combinations of fluids and substrates. The clustering accuracy ranges from 62 to 80%, compared to an accuracy by random assignment of 3 or 4%. The algorithms were also remarkably accurate at determining the presence or absence of biotin and streptavidin respectively in the liquid and on the glass, the salt composition, or the pH of the solution.

Original languageEnglish (US)
Pages (from-to)50-57
Number of pages8
JournalAnalytical Methods
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2012

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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