Identification of human polo-like kinase 1 as a potential therapeutic target in pancreatic cancer

Phillip J. Gray, David J. Bearss, Haiyong Han, Raymond Nagle, Ming Sound Tsao, Nicholas Dean, Daniel D. Von Hoff

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Pancreas cancer is the fourth leading cause of cancer-related death in adults in the United States. New molecular targets for diagnosis and therapy of this disease are desperately needed. In this study, we report on the mitotic serine-threonine kinase polo-like kinase 1 (Pik1) in pancreatic cancer. Plk1 mRNA was found to be overexpressed in 9 of 10 tested pancreatic cancer cell lines and in 4 of 4 tested human tumors. Immunohistochemical staining of a pancreatic tissue microarray showed that 26 of the 35 tumors taken directly from patients overexpressed Plk1. We also examined the effects of depleting Plk1 in pancreatic cancer cells by the use of antisense oligonucleotides. Antisense-treated pancreatic cancer cells showed cell cycle arrest in G2-M as well as a drastic reduction in proliferation rates. These data suggest that Plk1 is a potential therapeutic target in devising a treatment for patients with pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalMolecular Cancer Therapeutics
Volume3
Issue number5
StatePublished - May 1 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Identification of human polo-like kinase 1 as a potential therapeutic target in pancreatic cancer'. Together they form a unique fingerprint.

  • Cite this

    Gray, P. J., Bearss, D. J., Han, H., Nagle, R., Tsao, M. S., Dean, N., & Von Hoff, D. D. (2004). Identification of human polo-like kinase 1 as a potential therapeutic target in pancreatic cancer. Molecular Cancer Therapeutics, 3(5), 641-646.