Identification of isolate-specific sporozoite proteins of Cryptosporidium parvum by two-dimensional gel electrophoresis

J. R. Mead, R. C. Humphreys, D. W. Sammons, Charles R Sterling

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Five isolates of Cryptosporidium parvum collected from human, horse, and calf sources were compared for differences in sporozoite protein patterns by using two-dimensional gel electrophoresis. Silver-stained two-dimensional gels contained over 300 protein spots from detergent-solubilized sporozoites. A distinguishing 106-kilodalton peptide that shifted in isoelectric point was detected in four of the five isolates. Computerized two-dimensional gel analysis was performed to obtain objective quantitation of the pI shift. Three of these four isolates could be differentiated from one other by the pI shift in this peptide. The fifth isolate was distinguished by the absence of the 106-kilodalton peptide and the presence of a 40-kilodalton peptide that was not observed in any other isolate.

Original languageEnglish (US)
Pages (from-to)2071-2075
Number of pages5
JournalInfection and Immunity
Volume58
Issue number7
StatePublished - 1990

Fingerprint

Cryptosporidium parvum
Sporozoites
Electrophoresis, Gel, Two-Dimensional
Gels
Peptides
Isoelectric Point
Silver
Detergents
Horses
Proteins
peptide 106

ASJC Scopus subject areas

  • Immunology

Cite this

Identification of isolate-specific sporozoite proteins of Cryptosporidium parvum by two-dimensional gel electrophoresis. / Mead, J. R.; Humphreys, R. C.; Sammons, D. W.; Sterling, Charles R.

In: Infection and Immunity, Vol. 58, No. 7, 1990, p. 2071-2075.

Research output: Contribution to journalArticle

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