Identification of novel stress-responsive transcription factor genes in rice by cDNA array analysis

Cong Qing Wu, Hong Hong Hu, Ya Zeng, Da Cheng Liang, Ka Bin Xie, Jianwei - Zhang, Zhao Hui Chu, Li Zhong Xiong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Numerous studies have shown that array of transcription factors has a role in regulating plant responses to environmental stresses. Only a small portion of them however, have been identified or characterized. More than 2 300 putative transcription factors were predicted in the rice genome and more than half of them were supported by expressed sequences. With an attempt to identify novel transcription factors involved in the stress responses, a cDNA array containing 753 putative rice transcription factors was generated to analyze the transcript profiles of these genes under drought and salinity stresses and abscisic acid treatment at seedling stage of rice. About 80% of these transcription factors showed detectable levels of transcript in seedling leaves. A total of 18 up-regulated transcription factors and 29 down-regulated transcription factors were detected with the folds of changes from 2.0 to 20.5 in at least one stress treatment. Most of these stress-responsive genes have not been reported and the expression patterns for five genes under stress conditions were further analyzed by RNA gel blot analysis. These novel stress-responsive transcription factors provide new opportunities to study the regulation of gene expression in plants under stress conditions.

Original languageEnglish (US)
Pages (from-to)1216-1224
Number of pages9
JournalJournal of Integrative Plant Biology
Volume48
Issue number10
DOIs
StatePublished - Oct 2006
Externally publishedYes

Fingerprint

Oligonucleotide Array Sequence Analysis
Transcription Factors
transcription factors
Complementary DNA
Genes
rice
genes
Seedlings
Plant Gene Expression Regulation
Abscisic Acid
seedlings
Oryza
Drought
Droughts
Salinity
gene expression regulation
acid treatment
Gene expression
salt stress
plant response

Keywords

  • Abiotic stress cDNA array
  • Oryza sativa
  • Transcription factor

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Identification of novel stress-responsive transcription factor genes in rice by cDNA array analysis. / Wu, Cong Qing; Hu, Hong Hong; Zeng, Ya; Liang, Da Cheng; Xie, Ka Bin; Zhang, Jianwei -; Chu, Zhao Hui; Xiong, Li Zhong.

In: Journal of Integrative Plant Biology, Vol. 48, No. 10, 10.2006, p. 1216-1224.

Research output: Contribution to journalArticle

Wu, Cong Qing ; Hu, Hong Hong ; Zeng, Ya ; Liang, Da Cheng ; Xie, Ka Bin ; Zhang, Jianwei - ; Chu, Zhao Hui ; Xiong, Li Zhong. / Identification of novel stress-responsive transcription factor genes in rice by cDNA array analysis. In: Journal of Integrative Plant Biology. 2006 ; Vol. 48, No. 10. pp. 1216-1224.
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AU - Zhang, Jianwei -

AU - Chu, Zhao Hui

AU - Xiong, Li Zhong

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