Identification of prolactin-like proteins synthesized by normal murine lymphocytes

David W Montgomery, Jane A. LeFevre, Ellen D. Ulrich, Cynthia R. Adamson, Charles F. Zukoski

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Abstract

Cultured murine lymphoid cells release a PRL-like immunoreactive (IR) protein which may be important in immunity, as anti-PRL antisera inhibit lymphocyte proliferation in vitro. We examined culture supernatants (SNs) and cell lysates from concanavalin A (Con A) activated murine thymocytes to identify these proteins. Western blot analysis of cell lysates revealed three specifically-stained PRL-IRs. A doublet of bands at 35.6 and 33.6 kDa was associated with the particulate fraction of the cell. These PRL-IRs were present in lymphocytes independently of mitogen stimulation. In contrast, a 22 kDa PRL-IR was only produced in mitogen stimulated cells, and was specifically immunoprecipitated with anti-PRL antiserum. In addition, all three PRL-like IRs incorporated 35S-methionine in vitro, indicating that they are synthesized by these cells. Only the 22 kDa PRL-like protein was present in culture medium from stimulated cells, suggesting that this may be the PRL bioactivity previously demonstrated in SNs from murine lymphocytes.

Original languageEnglish (US)
Pages (from-to)2601-2603
Number of pages3
JournalEndocrinology
Volume127
Issue number5
StatePublished - Nov 1990

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Prolactin
Lymphocytes
Proteins
Mitogens
Immune Sera
Thymocytes
Concanavalin A
Methionine
Culture Media
Immunity
Cell Culture Techniques
Western Blotting
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Montgomery, D. W., LeFevre, J. A., Ulrich, E. D., Adamson, C. R., & Zukoski, C. F. (1990). Identification of prolactin-like proteins synthesized by normal murine lymphocytes. Endocrinology, 127(5), 2601-2603.

Identification of prolactin-like proteins synthesized by normal murine lymphocytes. / Montgomery, David W; LeFevre, Jane A.; Ulrich, Ellen D.; Adamson, Cynthia R.; Zukoski, Charles F.

In: Endocrinology, Vol. 127, No. 5, 11.1990, p. 2601-2603.

Research output: Contribution to journalArticle

Montgomery, DW, LeFevre, JA, Ulrich, ED, Adamson, CR & Zukoski, CF 1990, 'Identification of prolactin-like proteins synthesized by normal murine lymphocytes', Endocrinology, vol. 127, no. 5, pp. 2601-2603.
Montgomery DW, LeFevre JA, Ulrich ED, Adamson CR, Zukoski CF. Identification of prolactin-like proteins synthesized by normal murine lymphocytes. Endocrinology. 1990 Nov;127(5):2601-2603.
Montgomery, David W ; LeFevre, Jane A. ; Ulrich, Ellen D. ; Adamson, Cynthia R. ; Zukoski, Charles F. / Identification of prolactin-like proteins synthesized by normal murine lymphocytes. In: Endocrinology. 1990 ; Vol. 127, No. 5. pp. 2601-2603.
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