Identification of prolactin receptors in hepatic nuclei

Arthur R. Buckley; David W. Montgomery; Mary J.C. Hendrix; Charles F. Zukoski; Charles W. Putnam

doi:10.1016/0003-9861(92)90563-C

Identification of prolactin receptors in hepatic nuclei

Arthur R. Buckley, David W. Montgomery, Mary J.C. Hendrix, Charles F. Zukoski, Charles W. Putnam

Nursing, College of

Research output: Contribution to journal › Article

29 Scopus citations

Abstract

Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity ¹²⁵I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

Original language	English (US)
Pages (from-to)	198-206
Number of pages	9
Journal	Archives of Biochemistry and Biophysics
Volume	296
Issue number	1
DOIs	https://doi.org/10.1016/0003-9861(92)90563-C
State	Published - Jul 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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Buckley, A. R., Montgomery, D. W., Hendrix, M. J. C., Zukoski, C. F., & Putnam, C. W. (1992). Identification of prolactin receptors in hepatic nuclei. Archives of Biochemistry and Biophysics, 296(1), 198-206. https://doi.org/10.1016/0003-9861(92)90563-C

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abstract = "Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.",

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AB - Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

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