Identification of prolactin receptors in hepatic nuclei

Arthur R. Buckley, David W. Montgomery, Mary J.C. Hendrix, Charles F. Zukoski, Charles W. Putnam

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Prolactin is a trophic hormone which may act directly at the hepatocyte nucleus. In this study, specific prolactin binding sites were sought in purified rat liver nuclei. Saturable and specific, high affinity 125I-prolactin binding sites were demonstrated to be on or within the nucleus. Prolactin binding was competitively inhibited by rat and ovine prolactins but not by rat growth hormone. Using immunogold electron microscopy, we detected prolactin receptors throughout the nucleus, in association with heterochromatin. Furthermore, endogenous immunoreactive prolactin was demonstrated to be within hepatic nuclei. We conclude that rat liver nuclei possess prolactin binding sites which likely participate in hormone-directed growth processes.

Original languageEnglish (US)
Pages (from-to)198-206
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume296
Issue number1
DOIs
StatePublished - Jul 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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