Identification of the Maize chlorotic mottle virus capsid protein cistron and characterization of its subgenomic messenger RNA

S. A. Lommel, T. L. Kendall, Zhongguo Xiong, R. C. Nutter

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Maize chlorotic mottle virus (MCMV) is a 30-nm icosahedral plant virus composed of a single 25-kDa capsid protein component and a 4.4-kb single-stranded, positive-sense genomic RNA. Northern blot hybridization analysis detected a single 3′-terminal 1.1-kb subgenomic RNA in infected plants. Virion RNA directs the synthesis of several polypeptides in a rabbit reticulocyte lysate in vitro translation system of which only the 25-kDa polypeptide is immunoprecipitated by MCMV capsid protein antiserum. The 1.1-kb subgenomic RNA is a highly efficient messenger RNA for capsid protein synthesis. Positive polarity in vitro transcripts from 3′-proximal MCMV cDNA clones direct the synthesis of the capsid protein in in vitro translation experiments. These data suggest that the MCMV capsid protein is expressed from a subgenomic RNA in vivo, and that the 25-kDa capsid protein is encoded by the 3′-proximal open reading frame in the MCMV genome.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalVirology
Volume181
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Capsid Proteins
Zea mays
Viruses
Messenger RNA
RNA
Genes
Plant Viruses
Peptides
Reticulocytes
Northern Blotting
Virion
Open Reading Frames
Immune Sera
Complementary DNA
Clone Cells
Genome
Rabbits
In Vitro Techniques

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Identification of the Maize chlorotic mottle virus capsid protein cistron and characterization of its subgenomic messenger RNA. / Lommel, S. A.; Kendall, T. L.; Xiong, Zhongguo; Nutter, R. C.

In: Virology, Vol. 181, No. 1, 1991, p. 382-385.

Research output: Contribution to journalArticle

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