Identification of the Maize chlorotic mottle virus capsid protein cistron and characterization of its subgenomic messenger RNA

S. A. Lommel, T. L. Kendall, Z. Xiong, R. C. Nutter

Research output: Contribution to journalArticle

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Abstract

Maize chlorotic mottle virus (MCMV) is a 30-nm icosahedral plant virus composed of a single 25-kDa capsid protein component and a 4.4-kb single-stranded, positive-sense genomic RNA. Northern blot hybridization analysis detected a single 3′-terminal 1.1-kb subgenomic RNA in infected plants. Virion RNA directs the synthesis of several polypeptides in a rabbit reticulocyte lysate in vitro translation system of which only the 25-kDa polypeptide is immunoprecipitated by MCMV capsid protein antiserum. The 1.1-kb subgenomic RNA is a highly efficient messenger RNA for capsid protein synthesis. Positive polarity in vitro transcripts from 3′-proximal MCMV cDNA clones direct the synthesis of the capsid protein in in vitro translation experiments. These data suggest that the MCMV capsid protein is expressed from a subgenomic RNA in vivo, and that the 25-kDa capsid protein is encoded by the 3′-proximal open reading frame in the MCMV genome.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalVirology
Volume181
Issue number1
DOIs
StatePublished - Mar 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Virology

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