Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

Patrick J. Krysan, Jeff C. Young, Frans Tax, Michael R. Sussman

Research output: Contribution to journalArticle

227 Scopus citations

Abstract

The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.

Original languageEnglish (US)
Pages (from-to)8145-8150
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number15
DOIs
StatePublished - Jul 23 1996
Externally publishedYes

Keywords

  • polymerase chain reaction
  • protein kinase
  • reverse genetics

ASJC Scopus subject areas

  • General

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