Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

Patrick J. Krysan; Jeff C. Young; Frans Tax; Michael R. Sussman

doi:10.1073/pnas.93.15.8145

Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport

Patrick J. Krysan, Jeff C. Young, Frans Tax, Michael R. Sussman

Research output: Contribution to journal › Article › peer-review

227 Scopus citations

Abstract

The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.

Original language	English (US)
Pages (from-to)	8145-8150
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	93
Issue number	15
DOIs	https://doi.org/10.1073/pnas.93.15.8145
State	Published - Jul 23 1996
Externally published	Yes

Keywords

polymerase chain reaction
protein kinase
reverse genetics

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.93.15.8145

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Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. / Krysan, Patrick J.; Young, Jeff C.; Tax, Frans; Sussman, Michael R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 15, 23.07.1996, p. 8145-8150.

Research output: Contribution to journal › Article › peer-review

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