Abstract
The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8145-8150 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 93 |
| Issue number | 15 |
| DOIs | |
| State | Published - Jul 23 1996 |
| Externally published | Yes |
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Keywords
- polymerase chain reaction
- protein kinase
- reverse genetics
ASJC Scopus subject areas
- Genetics
- General
Cite this
Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport. / Krysan, Patrick J.; Young, Jeff C.; Tax, Frans; Sussman, Michael R.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 15, 23.07.1996, p. 8145-8150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport
AU - Krysan, Patrick J.
AU - Young, Jeff C.
AU - Tax, Frans
AU - Sussman, Michael R.
PY - 1996/7/23
Y1 - 1996/7/23
N2 - The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.
AB - The transferred DNA (T-DNA) of Agrobacterium tumefaciens serves as an insertional mutagen once integrated into a host plant's genome. As a means of facilitating reverse genetic analysis in Arabidopsis thaliana, we have developed a method that allows one to search for plants carrying T-DNA insertions within any sequenced Arabidopsis gene. Using PCR, we screened a collection of 9100 independent T-DNA-transformed Arabidopsis lines and found 17 T-DNA insertions within the 63 genes analyzed. The genes surveyed include members of various gene families involved in signal transduction and ion transport. As an example, data are shown for a T-DNA insertion that was found within CPK-9, a member of the gene family encoding calmodulin-domain protein kinases.
KW - polymerase chain reaction
KW - protein kinase
KW - reverse genetics
UR - http://www.scopus.com/inward/record.url?scp=0029821246&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029821246&partnerID=8YFLogxK
U2 - 10.1073/pnas.93.15.8145
DO - 10.1073/pnas.93.15.8145
M3 - Article
C2 - 8755618
AN - SCOPUS:0029821246
VL - 93
SP - 8145
EP - 8150
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 15
ER -