Identification of two novel functional p53 responsive elements in the herpes simplex virus-1 genome

Jui Cheng Hsieh, Ryan Kuta, Courtney R. Armour, Paul E. Boehmer

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Analysis of the herpes simplex virus-1 (HSV-1) genome reveals two candidate p53 responsive elements (p53RE), located in proximity to the replication origins oriL and oriS, referred to as p53RE-L and p53RE-S, respectively. The sequences of p53RE-L and p53RE-S conform to the p53 consensus site and are present in HSV-1 strains KOS, 17, and F. p53 binds to both elements in vitro and in virus-infected cells. Both p53RE-L and p53RE-S are capable of conferring p53-dependent transcriptional activation onto a heterologous reporter gene. Importantly, expression of the essential immediate early viral transactivator ICP4 and the essential DNA replication protein ICP8, that are adjacent to p53RE-S and p53RE-L, are repressed in a p53-dependent manner. Taken together, this study identifies two novel functional p53RE in the HSV-1 genome and suggests a complex mechanism of viral gene regulation by p53 which may determine progression of the lytic viral replication cycle or the establishment of latency.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalVirology
Volume460-461
Issue number1
DOIs
StatePublished - Jul 2014

Keywords

  • Gene expression
  • Herpes simplex virus 1
  • P53
  • Responsive elements

ASJC Scopus subject areas

  • Virology

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