Identification of zinc finger binding protein 89 (ZBP-89) as a transcriptional activator for a major bovine growth hormone receptor promoter

Qingfu Xu, Lynn Springer, Juanita L. Merchant, Honglin Jiang

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The objective of this study was to identify the transcription factors that regulate the expression of growth hormone receptor (GHR) 1A mRNA, a major GHR mRNA variant in the bovine liver. A deoxyribonuclease I footprint analysis revealed that the GHR1A promoter region -69 to -30 (relative to the transcription start site for GHR1A mRNA) contained binding sites for bovine liver nuclear proteins. Using a yeast one-hybrid analysis, zinc finger binding protein 89 (ZBP-89) was identified as a binding protein to this promoter region. Binding of ZBP-89 to the GHR1A promoter region -69 to -30 was further confirmed by an electrophoretic mobility shift assay. In cotransfection analyses, overexpression of ZBP-89 enhanced (P < 0.01) the activity of the GHR1A promoter and this enhancement was dependent on the putative ZBP-89 binding site in the promoter. These results together indicate that ZBP-89 is a transcription factor that regulates the expression of GHR1A mRNA.

Original languageEnglish (US)
Pages (from-to)88-95
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume251
Issue number1-2
DOIs
StatePublished - Jun 7 2006
Externally publishedYes

Keywords

  • Growth hormone receptor
  • Promoter
  • Transcription factors
  • ZBP-89

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint

Dive into the research topics of 'Identification of zinc finger binding protein 89 (ZBP-89) as a transcriptional activator for a major bovine growth hormone receptor promoter'. Together they form a unique fingerprint.

Cite this