IgE regulation and lymphokine patterns in aging humans

Hassan Al-Rayes, William Pachas, Nadim Mirza, Deborah J. Ahern, Raif S. Geha, Donata Vercelli

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

IgE production declines with age, and allergic symptoms tend to improve. Aging therefore represents an in vivo model to study IgE regulation. We compared IgE production in older (≥ 60 years old) and young (15 to 30 years old) nonatopic individuals. Addition of exogenous interleukin-4 (IL-4) to mononuclear cells from older and young subjects induced equivalent amounts of IgE, indicating that IL-4 responsiveness is preserved in aging. After surface receptor stimulation with concanavalin A, IL-4 production by mononuclear cells from older subjects was approximately 50% as compared with the young, whereas interferon-γ (IFN-γ) production was reduced threefold (p = 0.008). By contrast, stimulation with phorbol esters and ionophore, which bypass surface receptor signaling, induced comparable amounts of IL-4 and IFN-γ in older and young subjects. These data point to an impairment in T-cell membrane signal transduction in older individuals. This hypothesis was directly confirmed by showing that Ca+2 fluxes after CD3 crosslinking were significantly (p = 0.014) decreased in the older population. Our findings altogether suggest that an age-dependent T-cell activation defect may result in decreased availability of IL-4 and in the waning of IgE responses.

Original languageEnglish (US)
Pages (from-to)630-636
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume90
Issue number4 PART 1
DOIs
StatePublished - Oct 1992
Externally publishedYes

Keywords

  • IFN-γ
  • IL-4
  • IgE
  • aging
  • calcium fluxes
  • lymphokines

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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