Imaging neural activity with temporal and cellular resolution using FISH

J. F. Guzowski, B. L. McNaughton, Carol A Barnes, P. F. Worley

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Immediate early genes have gained widespread use as neural activity markers in studies of brain function. The recent development of cellular compartment analysis of temporal activity, which combines sensitive fluorescence in situ hybridization and laser scanning confocal microscopy, overcomes the lack of temporal resolution of standard methodologies and allows the tracking of distinct steps in the synthesis and processing of immediate early gene RNAs. Thus, this technique provides information about when individual neurons are activated and allows the visualization, within a single brain, of different neuronal populations engaged by two distinct experiences.

Original languageEnglish (US)
Pages (from-to)579-584
Number of pages6
JournalCurrent Opinion in Neurobiology
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2001

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Immediate-Early Genes
Brain
Fluorescence In Situ Hybridization
Confocal Microscopy
RNA
Neurons
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Imaging neural activity with temporal and cellular resolution using FISH. / Guzowski, J. F.; McNaughton, B. L.; Barnes, Carol A; Worley, P. F.

In: Current Opinion in Neurobiology, Vol. 11, No. 5, 01.10.2001, p. 579-584.

Research output: Contribution to journalArticle

Guzowski, J. F. ; McNaughton, B. L. ; Barnes, Carol A ; Worley, P. F. / Imaging neural activity with temporal and cellular resolution using FISH. In: Current Opinion in Neurobiology. 2001 ; Vol. 11, No. 5. pp. 579-584.
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