Imaging of tumor necroses using full-frame optical coherence imaging

P. Yu, Leilei Peng, M. Mustata, D. D. Nolte, J. J. Turek, M. R. Melloch, C. Dunsby, Y. Gu, P. M W French

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Holographic optical coherence imaging (OCI) has been used to acquire depth resolved images in tumor spheroids. OCI is a coherence-domain imaging technique that uses dynamic holography as the coherence gate. The technique is full-frame (en face) and background free, allowing real-time acquisition to a digital camera without motional reconstruction artifacts. We describe the method of operation of the holographic OCI on highly scattering specimens of tumor spheroids. Because of the sub-resolution structure in the sample, the holograms consist primarily of speckle fields. We present two kinds of volumetric data acquisition. One is uses fly-throughs with a stepping reference delay. Another is static holograms at a fixed reference delay with the coherence gate inside the tumor spheroids. At a fixed reference delay, the holograms consist of time-dependent speckle patterns. The method can be used to study cell motility inside tumor spheroids when metabolic or cross-linking poisons are delivered to the specimens.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsV.V. Tuchin, J.A. Izatt, J.G. Fujimoto
Pages34-41
Number of pages8
Volume4956
DOIs
StatePublished - 2003
Externally publishedYes
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII - San Jose, CA, United States
Duration: Jan 27 2003Jan 29 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII
CountryUnited States
CitySan Jose, CA
Period1/27/031/29/03

Fingerprint

necrosis
Tumors
tumors
spheroids
Imaging techniques
Holograms
Speckle
poisons
locomotion
digital cameras
Holography
speckle patterns
Digital cameras
imaging techniques
holography
data acquisition
artifacts
Data acquisition
acquisition
Scattering

Keywords

  • 3D imaging
  • Necrosis
  • Optical coherence imaging
  • Tumor spheroids

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Yu, P., Peng, L., Mustata, M., Nolte, D. D., Turek, J. J., Melloch, M. R., ... French, P. M. W. (2003). Imaging of tumor necroses using full-frame optical coherence imaging. In V. V. Tuchin, J. A. Izatt, & J. G. Fujimoto (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4956, pp. 34-41) https://doi.org/10.1117/12.477823

Imaging of tumor necroses using full-frame optical coherence imaging. / Yu, P.; Peng, Leilei; Mustata, M.; Nolte, D. D.; Turek, J. J.; Melloch, M. R.; Dunsby, C.; Gu, Y.; French, P. M W.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / V.V. Tuchin; J.A. Izatt; J.G. Fujimoto. Vol. 4956 2003. p. 34-41.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yu, P, Peng, L, Mustata, M, Nolte, DD, Turek, JJ, Melloch, MR, Dunsby, C, Gu, Y & French, PMW 2003, Imaging of tumor necroses using full-frame optical coherence imaging. in VV Tuchin, JA Izatt & JG Fujimoto (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4956, pp. 34-41, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, San Jose, CA, United States, 1/27/03. https://doi.org/10.1117/12.477823
Yu P, Peng L, Mustata M, Nolte DD, Turek JJ, Melloch MR et al. Imaging of tumor necroses using full-frame optical coherence imaging. In Tuchin VV, Izatt JA, Fujimoto JG, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4956. 2003. p. 34-41 https://doi.org/10.1117/12.477823
Yu, P. ; Peng, Leilei ; Mustata, M. ; Nolte, D. D. ; Turek, J. J. ; Melloch, M. R. ; Dunsby, C. ; Gu, Y. ; French, P. M W. / Imaging of tumor necroses using full-frame optical coherence imaging. Proceedings of SPIE - The International Society for Optical Engineering. editor / V.V. Tuchin ; J.A. Izatt ; J.G. Fujimoto. Vol. 4956 2003. pp. 34-41
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