Imaging of tumor necroses using full-frame optical coherence imaging

P. Yu, L. L. Peng, M. Mustata, D. D. Nolte, J. J. Turek, M. R. Melloch, C. Dunsby, Y. Gu, P. M.W. French

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

Holographic optical coherence imaging (OCI) has been used to acquire depth resolved images in tumor spheroids. OCI is a coherence-domain imaging technique that uses dynamic holography as the coherence gate. The technique is full-frame (en face) and background free, allowing real-time acquisition to a digital camera without motional reconstruction artifacts. We describe the method of operation of the holographic OCI on highly scattering specimens of tumor spheroids. Because of the sub-resolution structure in the sample, the holograms consist primarily of speckle fields. We present two kinds of volumetric data acquisition. One is uses fly-throughs with a stepping reference delay. Another is static holograms at a fixed reference delay with the coherence gate inside the tumor spheroids. At a fixed reference delay, the holograms consist of time-dependent speckle patterns. The method can be used to study cell motility inside tumor spheroids when metabolic or cross-linking poisons are delivered to the specimens.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4956
DOIs
StatePublished - Dec 5 2003
Externally publishedYes
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII - San Jose, CA, United States
Duration: Jan 27 2003Jan 29 2003

Keywords

  • 3D imaging
  • Necrosis
  • Optical coherence imaging
  • Tumor spheroids

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Yu, P., Peng, L. L., Mustata, M., Nolte, D. D., Turek, J. J., Melloch, M. R., Dunsby, C., Gu, Y., & French, P. M. W. (2003). Imaging of tumor necroses using full-frame optical coherence imaging. Proceedings of SPIE - The International Society for Optical Engineering, 4956, 34-41. https://doi.org/10.1117/12.477823