Imaging stented tissue engineered blood vessel mimics

Garret T. Bonnema, Kristen O. Cardinal, Stuart K. Williams, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An ideal vascular stent design promotes a thin anti-thrombogenic cellular lining while avoiding restenosis. To assess the utility of their designs, stent manufactures often use destructive techniques such as scanning electron microscopy to measure the percentage of the stent covered with a cellular lining. In this study, we use a custom-built longitudinal/rotational scanning endoscope and determine the ability of optical coherence tomography (OCT) to quantify the percent cellular coverage of stented tissue engineered blood vessel mimics. Stents were deployed within twelve mimics after 14-day s of development in bioreactors. OCT images were acquired within the bioreactor at several time points after the stent deployment. At 20-days post deployment, the mimics were fixed and imaged volumetrically with OCT. Matlab software was developed to automatically calculate the percent cellular coverage from the OCT images. Algorithm results were compared to similar measurements performed with bis-benzimide (BBI) fluorescence imaging and manually calculated percent coverage from three different observers of the OCT images. Progressive accumulation of cellular material on the stents could be visualized with OCT. For the volumetric images, the algorithm calculated percent cellular coverages ranging from 11 to 76%. Good agreement was found between the OCT-based measurements and the other techniques. On average, the algorithm differed less than 5% from the manual percent coverage calculations. OCT together with automated software can provide an accurate, non-destructive measurement of the percent cellular coverage of vascular stents.

Original languageEnglish (US)
Title of host publicationOptics in Tissue Engineering and Regenerative Medicine II
DOIs
StatePublished - Jun 26 2008
EventOptics in Tissue Engineering and Regenerative Medicine II - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6858
ISSN (Print)1605-7422

Other

OtherOptics in Tissue Engineering and Regenerative Medicine II
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Bonnema, G. T., Cardinal, K. O., Williams, S. K., & Barton, J. K. (2008). Imaging stented tissue engineered blood vessel mimics. In Optics in Tissue Engineering and Regenerative Medicine II [68580F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6858). https://doi.org/10.1117/12.763991