Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

Michael J. Danilich, Kandice Kottke-Marchant, Dominic F Gervasio, Tracey L. Bonfield, James M. Anderson, Roger E. Marchant

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.

Original languageEnglish (US)
Title of host publicationTransactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium
PublisherPubl by Soc for Biomaterials
Pages132
Number of pages1
Volume14
StatePublished - 1991
Externally publishedYes
Event17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium - Scottsdale, AZ, USA
Duration: May 1 1991May 5 1991

Other

Other17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium
CityScottsdale, AZ, USA
Period5/1/915/5/91

Fingerprint

Glucose Oxidase
Glucose oxidase
Plasmas
Polyurethanes
Octoxynol
Sodium dodecyl sulfate
Sodium Dodecyl Sulfate
Surface properties
Polymers
Phosphates
Coloring Agents
Sodium
Molecules
2-pyrrolidone

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Danilich, M. J., Kottke-Marchant, K., Gervasio, D. F., Bonfield, T. L., Anderson, J. M., & Marchant, R. E. (1991). Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium (Vol. 14, pp. 132). Publ by Soc for Biomaterials.

Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). / Danilich, Michael J.; Kottke-Marchant, Kandice; Gervasio, Dominic F; Bonfield, Tracey L.; Anderson, James M.; Marchant, Roger E.

Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14 Publ by Soc for Biomaterials, 1991. p. 132.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Danilich, MJ, Kottke-Marchant, K, Gervasio, DF, Bonfield, TL, Anderson, JM & Marchant, RE 1991, Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). in Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. vol. 14, Publ by Soc for Biomaterials, pp. 132, 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA, 5/1/91.
Danilich MJ, Kottke-Marchant K, Gervasio DF, Bonfield TL, Anderson JM, Marchant RE. Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). In Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14. Publ by Soc for Biomaterials. 1991. p. 132
Danilich, Michael J. ; Kottke-Marchant, Kandice ; Gervasio, Dominic F ; Bonfield, Tracey L. ; Anderson, James M. ; Marchant, Roger E. / Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. Vol. 14 Publ by Soc for Biomaterials, 1991. pp. 132
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