Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea)

Michael J. Danilich, Kandice Kottke-Marchant, Dominic Gervasio, Tracey L. Bonfield, James M. Anderson, Roger E. Marchant

Research output: Contribution to conferencePaper

Abstract

In this paper, the combination of the excellent bulk properties of a segmented polyurethane and the excellent surface properties of a RF plasma polymer for the immobilization of a bioactive molecule is discussed. Specifically, glucose oxidase (GOx) has been covalently immobilized to poly(etherurethaneurea) (PEUU) films which were first modified with RF plasma polymerized N-vinyl-2-pyrrolidone (PPNVP). The results of the RIA and the immunochemical stain indicated that washes based on published procedures were inadequate in removing physically adsorbed GOx from the surfaces of PEUU and PPNVP/PEUU controls. The only wash which was effective consisted of continuous magnetic stirring in 900 ml of 2% sodium dodecyl sulfate, 2% Triton X-100, and 20 mM sodium phosphate (pH 7.0) for 20-24 hrs. each at 5°C.

Original languageEnglish (US)
Number of pages1
StatePublished - Jan 1 1991
Externally publishedYes
Event17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium - Scottsdale, AZ, USA
Duration: May 1 1991May 5 1991

Other

Other17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium
CityScottsdale, AZ, USA
Period5/1/915/5/91

ASJC Scopus subject areas

  • Materials Science(all)

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    Danilich, M. J., Kottke-Marchant, K., Gervasio, D., Bonfield, T. L., Anderson, J. M., & Marchant, R. E. (1991). Immobilization of glucose oxidase onto radiofrequency plasma modified poly(etherurethaneurea). Paper presented at 17th Annual Meeting of the Society for Biomaterials in conjunction with the 23rd International Biomaterials Symposium, Scottsdale, AZ, USA, .