Immortalized connexin43 knockout cell lines display a subset of biological properties associated with the transformed phenotype

Kendra Dean Martyn, Wendy E. Kurata, Bonnie J. Warn-Cramer, Janis M Burt, Erica TenBroek, Alan F. Lau

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Abstract

Immortalized cells from embryonic connexin43 knockout mice (Cx43(-/-)) and homozygous littermates (Cx43(+/+)) were cloned and characterized to determine whether the absence of Cx43 function would induce observable phenotypic changes. Cells of the Cx43(+/+) clones expressed Cx43 and engaged in gap junctional communication with 10-12 neighboring cells. The Cx43(-/-) cells were devoid of Cx43 and communicated to less than 1 cell. Electrophysiological analysis indicated that the Cx43(-/-) cells communicated through Cx45 channels from 8-80-fold less than did the Cx43(+/+) subclones, which seemed to communicate through Cx43 and Cx45 channels. The Cx43(-/-) clones grew at faster rates and to higher saturation densities, had a more spindly morphology, were more refractile, and adhered less well to the substratum than did the Cx43(+/+) clones. Reintroducing the Cx43 gene into the Cx43(-/-) clones resulted in three subclones that communicated to 3-4 cells. Partial restoration of gap junctional communication in the three subclones was accompanied by reduced growth rates and saturation densities (2-fold compared to that of parental Cx43(-/-) clones) but no reversions in morphology or cell-substratum adhesion. The increased growth rates and saturation densities, altered morphology, and decreased cell adhesion displayed by the Cx43(-/-) clones reflect a subset of the properties of transformed cells. These studies advance the hypothesis that loss of Cx43 function during development may cause cells to acquire a preneoplastic condition.

Original languageEnglish (US)
Pages (from-to)1015-1027
Number of pages13
JournalCell Growth and Differentiation
Volume8
Issue number9
StatePublished - Sep 1997

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Connexin 43
Phenotype
Cell Line
Clone Cells
Cell Adhesion
Communication
Precancerous Conditions

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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Immortalized connexin43 knockout cell lines display a subset of biological properties associated with the transformed phenotype. / Martyn, Kendra Dean; Kurata, Wendy E.; Warn-Cramer, Bonnie J.; Burt, Janis M; TenBroek, Erica; Lau, Alan F.

In: Cell Growth and Differentiation, Vol. 8, No. 9, 09.1997, p. 1015-1027.

Research output: Contribution to journalArticle

Martyn, Kendra Dean ; Kurata, Wendy E. ; Warn-Cramer, Bonnie J. ; Burt, Janis M ; TenBroek, Erica ; Lau, Alan F. / Immortalized connexin43 knockout cell lines display a subset of biological properties associated with the transformed phenotype. In: Cell Growth and Differentiation. 1997 ; Vol. 8, No. 9. pp. 1015-1027.
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