Immuno-gene therapy of melanoma by tumor antigen epitope modified IFN-γ

Xianghui He, Phoebe Luo, Tom C. Tsang, Tong Zhang, David T. Harris

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Cytokine-based vaccines play a major part in tumor immuno-gene therapy. However, down-regulated antigen expression on tumor cells may diminish the immuno-potentiating aspects of cellular vaccines. In this study, we coexpressed a tumor antigen epitope with IFN-γ in the same gene by replacing the IFN-γ signal peptide with an antigen epitope-expressing signal peptide. We then investigated the effect of the antigen epitope-incorporated IFN-γ on the immunotherapy of murine melanoma B16 tumors. Results showed that TRP-2 epitope-expressing IFN-γ decreased B16 tumorigenicity and enhanced its immunogenicity after gene transfer. Protective immunity against wild type B16 tumors was induced by vaccination with IFN-γ transiently gene-modified tumor cells. These data suggest that cellular vaccines engineered to express an antigen epitope within an immunostimulatory cytokine could potentiate the immunization effect.

Original languageEnglish (US)
Pages (from-to)741-749
Number of pages9
JournalCancer Immunology, Immunotherapy
Volume54
Issue number8
DOIs
StatePublished - Jul 1 2005

Keywords

  • Antigen epitope
  • Cytokine
  • Immunotherapy
  • Tumor
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

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