Immunoarchitecture of the regenerating rat spleen

effects of partial splenectomy and heterotopic autotransplantation.

M. C. Dugan, T. M. Grogan, Marlys H Witte, C. Rangel, L. Richter, C. L. Witte, D. B. Van Wyck

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To investigate the microstructure of in situ (eutopic) and autotransplanted (ectopic) splenic remnants, adult Sprague-Dawley rats were studied 60 days after subtotal (approximately 80%) splenectomy, total splenectomy followed by single or multiple remnant intraperitoneal autotransplantation, or sham operation. Total nucleated cell counts were determined in excised splenic remnants, and immunohistochemical staining using monoclonal antibodies to rat B- and T-cell antigens was performed in serial tissue sections. Immunoarchitecture of eutopic remnants was indistinguishable from that of intact spleens and total nucleated cell counts remained proportional to weight. In contrast, ectopic remnants showed sparsity and abnormal mixing of B and T lymphocyte subpopulations with widespread loss of follicles and periarteriolar lymphoid sheaths in addition to lower density and marked reduction of total nucleated cells. These findings provide immunohistologic evidence that preservation of intact vasculature is critical to splenic architecture, which may account in part for the demonstrable functional inferiority of ectopic remnants.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalScanning Electron Microscopy
Issue numberPt 2
StatePublished - 1986
Externally publishedYes

Fingerprint

T-cells
Autologous Transplantation
Splenectomy
Rats
Spleen
Cell Count
T-Lymphocytes
Monoclonal antibodies
Viral Tumor Antigens
Lymphocyte Subsets
Antigens
Sprague Dawley Rats
B-Lymphocytes
Monoclonal Antibodies
Tissue
Staining and Labeling
Weights and Measures
Microstructure

ASJC Scopus subject areas

  • Biophysics
  • Control and Systems Engineering

Cite this

Dugan, M. C., Grogan, T. M., Witte, M. H., Rangel, C., Richter, L., Witte, C. L., & Van Wyck, D. B. (1986). Immunoarchitecture of the regenerating rat spleen: effects of partial splenectomy and heterotopic autotransplantation. Scanning Electron Microscopy, (Pt 2), 607-613.

Immunoarchitecture of the regenerating rat spleen : effects of partial splenectomy and heterotopic autotransplantation. / Dugan, M. C.; Grogan, T. M.; Witte, Marlys H; Rangel, C.; Richter, L.; Witte, C. L.; Van Wyck, D. B.

In: Scanning Electron Microscopy, No. Pt 2, 1986, p. 607-613.

Research output: Contribution to journalArticle

Dugan, MC, Grogan, TM, Witte, MH, Rangel, C, Richter, L, Witte, CL & Van Wyck, DB 1986, 'Immunoarchitecture of the regenerating rat spleen: effects of partial splenectomy and heterotopic autotransplantation.', Scanning Electron Microscopy, no. Pt 2, pp. 607-613.
Dugan, M. C. ; Grogan, T. M. ; Witte, Marlys H ; Rangel, C. ; Richter, L. ; Witte, C. L. ; Van Wyck, D. B. / Immunoarchitecture of the regenerating rat spleen : effects of partial splenectomy and heterotopic autotransplantation. In: Scanning Electron Microscopy. 1986 ; No. Pt 2. pp. 607-613.
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