Immunochemical characterization of the Ly-8.2 murine lymphocyte alloantigen: Possible relationship to actin

T. L. Delovitch, A. Fegelman, B. H. Barber, Jeffrey A Frelinger

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An immunochemical analysis of the Ly-8.2 antigen was performed. Reactivity of B10-radiolabeled T and B cell lysates with a C3H anti-AKR serum immunoprecipitates a major 43,000 m.w. protein detectable by one-dimensional gel electrophoresis. This protein can be radioiodinated, however, it does not appear to bind to lentil lectin. Two-dimensional gel electrophoresis and peptide-mapping studies indicate that this protein is structurally very similar to lymphocyte actin. The possible molecular relationship between Ly-8.2, other lymphocyte membrane antigens, and actin is discussed.

Original languageEnglish (US)
Pages (from-to)326-333
Number of pages8
JournalJournal of Immunology
Volume122
Issue number1
StatePublished - 1979
Externally publishedYes

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Isoantigens
Actins
Lymphocytes
Ly Antigens
Proteins
Peptide Mapping
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
B-Lymphocytes
Gels
T-Lymphocytes
Antigens
Membranes
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Immunochemical characterization of the Ly-8.2 murine lymphocyte alloantigen : Possible relationship to actin. / Delovitch, T. L.; Fegelman, A.; Barber, B. H.; Frelinger, Jeffrey A.

In: Journal of Immunology, Vol. 122, No. 1, 1979, p. 326-333.

Research output: Contribution to journalArticle

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