Immunocytochemical detection of 1, 25-Dihydroxyvitamin d receptors in normal human tissues

U. Berger, P. Wilson, R. A. McClelland, K. Colston, M. R. Haussler, J. W. Pike, R. C. Coombes

Research output: Contribution to journalArticlepeer-review

185 Scopus citations

Abstract

We developed an immunocytochemical technique to visualize the receptors for 1, 25-dihydroxyvitamin D[1, 25-(OH)2D receptor] in cryostat sections of normal human tissues, using a rat monoclonal antibody (9A7γ) to the chick intestinal receptor, which has been found to react with mammalian 1, 25-(OH)2D receptors. Localization of the antigen was predominantly nuclear, with little cytoplasmic immunoreactivity. Specific staining was seen in the nuclei of many normal epithelial tissues, including liver, kidney, thyroid, adrenal, gastrointestinal tract, breast, and skin. No nuclear staining was seen when tissue sections were incubated with normal rat immunoglobulin G or when the monoclonal antibody was preincubated with a receptor-enriched chick intestinal cytosol preparation. Our results demonstrate that the receptor for 1, 25-(OH)2D is localized in the nucleus and widely distributed in normal human tissues.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume67
Issue number3
DOIs
StatePublished - Sep 1988

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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