Immunocytochemical Detection of 1, 25-Dihydroxyvitamin D3Receptor in Breast Cancer

Uta Berger, Patricia Wilson, Richard A. Mcclelland, Kay Colston, Mark R. Haussler, J. Wesley Pike, R. Charles Coombes

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Abstract

We have developed an immunocytochemical technique to visualize the receptor for 1, 25-dihydroxyvitamin D3[l, 25(OH>2D3] in cryostat sections of human breast tumors and normal human breast tissue utilizing a monoclonal antibody (9A7γ) to chick intestinal receptor which recognizes mammalian l, 25(OH)2D3receptor. Specific staining was observed in the nuclei of tumor cells. Previous studies by our group have shown that a high proportion of breast tumors bind radiolabeled l, 25(OH)2D3and we have confirmed this, demonstrating immunocytochemical 1, 25(Oh)2D3receptor in 43 of 55 (78%) of breast carcinomas. No correlation with the presence of immunostainable estrogen receptor was found in these breast cancer specimens. Sections of normal breast showed immunoreactivity in the nuclei of epithelial cells of the lobules and ducts. Our results demonstrate that the receptor for l, 25(OH)2D3resides predominantly in the nucleus of breast carcinoma cells. The reason for its prominent expression in breast cancers is not yet known but may be related to growth regulation.

Original languageEnglish (US)
Pages (from-to)6793-6799
Number of pages7
JournalCancer Research
Volume47
StatePublished - Dec 1987

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Berger, U., Wilson, P., Mcclelland, R. A., Colston, K., Haussler, M. R., Wesley Pike, J., & Charles Coombes, R. (1987). Immunocytochemical Detection of 1, 25-Dihydroxyvitamin D3Receptor in Breast Cancer. Cancer Research, 47, 6793-6799.