Immunocytochemical detection of 1,25-dihydroxyvitamin D receptors in normal human tissues

U. Berger, P. Wilson, R. A. McClelland, K. Colston, Mark R Haussler, J. W. Pike, R. C. Coombes

Research output: Contribution to journalArticle

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Abstract

We developed an immunocytochemical technique to visualize the receptors for 1,25-dihydroxyvitamin D [1,25-(OH)2D receptor] in cryostat sections of normal human tissues, using a rat monoclonal antibody (9A7γ) to the chick intestinal receptor, which has been found to react with mammalian 1,25-(OH)2D receptors. Localization of the antigen was predominantly nuclear, with little cytoplasmic immunoreactivity. Specific staining was seen in the nuclei of many normal epithelial tissues, including liver, kidney, thyroid, adrenal,gastrointestinal tract, breast, and skin. No nuclear staining was seen when tissue sections were incubated with normal rat immunoglobulin G or when the monoclonal antibody was preincubated with a receptor-enriched chick intestinal cytosol preparation. Our results demonstrate that the receptor for 1,25-(OH)2D is localized in the nucleus and widely distributed in normal human tissues.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume67
Issue number3
StatePublished - 1988

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Tissue
Monoclonal Antibodies
Staining and Labeling
Rats
Cytosol
Cryostats
Gastrointestinal Tract
Thyroid Gland
Breast
Epithelium
Immunoglobulin G
Liver
Kidney
Antigens
Skin
1,25-dihydroxyvitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Berger, U., Wilson, P., McClelland, R. A., Colston, K., Haussler, M. R., Pike, J. W., & Coombes, R. C. (1988). Immunocytochemical detection of 1,25-dihydroxyvitamin D receptors in normal human tissues. Journal of Clinical Endocrinology and Metabolism, 67(3), 607-613.

Immunocytochemical detection of 1,25-dihydroxyvitamin D receptors in normal human tissues. / Berger, U.; Wilson, P.; McClelland, R. A.; Colston, K.; Haussler, Mark R; Pike, J. W.; Coombes, R. C.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 67, No. 3, 1988, p. 607-613.

Research output: Contribution to journalArticle

Berger, U, Wilson, P, McClelland, RA, Colston, K, Haussler, MR, Pike, JW & Coombes, RC 1988, 'Immunocytochemical detection of 1,25-dihydroxyvitamin D receptors in normal human tissues', Journal of Clinical Endocrinology and Metabolism, vol. 67, no. 3, pp. 607-613.
Berger, U. ; Wilson, P. ; McClelland, R. A. ; Colston, K. ; Haussler, Mark R ; Pike, J. W. ; Coombes, R. C. / Immunocytochemical detection of 1,25-dihydroxyvitamin D receptors in normal human tissues. In: Journal of Clinical Endocrinology and Metabolism. 1988 ; Vol. 67, No. 3. pp. 607-613.
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