Immunocytochemical identification of Rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia

J. A. Reed, D. J. Brigati, Stuart D Flynn, N. S. McNutt, K. W. Min, D. F. Welch, L. N. Slater

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We report the immunocytochemical identification of Rochalimaea henselae, a newly recognized fastidious, Gram-negative, Warthin-Starry-positive organism, as the common pathogen in bacillary angiomatosis (BA), bacillary peliosis (BP) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. Immunogenic proteins of the R. henselae strain isolated from the blood of a febrile immunocompromised patient with BP of the liver were used to produce primary immune serum in rabbits. Using immunocytochemical procedures, the polyclonal antiserum reacted strongly not only with the immunizing strain of the bacteria, but also with other blood isolates of R. henselae (five cases) from both immunocompromised and immunocompetent patients and with the organisms present in the tissue lesions of cutaneous BA (five cases) and BP of the liver (two cases) and spleen (one case). The blood isolates and BA and BP tissue samples were obtained from widely separated geographic areas. The antiserum was weakly cross-reactive with cultures of Rochalimaea quintana, an organism closely related to R. henselae, but this reactivity was eliminated by specific adsorption. The antiserum did not cross-react with the Warthin-Starry-positive organisms associated with cat scratch disease (Afipia felis), syphilis (Treponema pallidum), Lyme disease (Borrelia burgdorferi) or chronic active gastritis (Helicobacter pylori). Likewise, the antiserum did not identify organisms in eight cases of Kaposi's sarcoma, a disorder of immunocompromised patients that is clinically similar to BA. Further studies are needed to determine the prevalence of this newly recognized organism as well as its possible involvement in other angioproliferative diseases.

Original languageEnglish (US)
Pages (from-to)650-657
Number of pages8
JournalAmerican Journal of Surgical Pathology
Volume16
Issue number7
StatePublished - 1992
Externally publishedYes

Fingerprint

Bacillary Angiomatosis
Bartonella henselae
Purpura
Bacteremia
Immunocompromised Host
Immune Sera
Fever
Afipia
Liver
Bartonella quintana
Spleen
Cat-Scratch Disease
Treponema pallidum
Borrelia burgdorferi
Lyme Disease
Kaposi's Sarcoma
Gastritis
Syphilis
Helicobacter pylori
Adsorption

Keywords

  • Acquired immunodeficiency syndrome
  • Bacillary angiomatosis
  • Bacillary peliosis
  • Bacteremia
  • Immunocytochemistry
  • Rochalimaea henselae

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Immunocytochemical identification of Rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia. / Reed, J. A.; Brigati, D. J.; Flynn, Stuart D; McNutt, N. S.; Min, K. W.; Welch, D. F.; Slater, L. N.

In: American Journal of Surgical Pathology, Vol. 16, No. 7, 1992, p. 650-657.

Research output: Contribution to journalArticle

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abstract = "We report the immunocytochemical identification of Rochalimaea henselae, a newly recognized fastidious, Gram-negative, Warthin-Starry-positive organism, as the common pathogen in bacillary angiomatosis (BA), bacillary peliosis (BP) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. Immunogenic proteins of the R. henselae strain isolated from the blood of a febrile immunocompromised patient with BP of the liver were used to produce primary immune serum in rabbits. Using immunocytochemical procedures, the polyclonal antiserum reacted strongly not only with the immunizing strain of the bacteria, but also with other blood isolates of R. henselae (five cases) from both immunocompromised and immunocompetent patients and with the organisms present in the tissue lesions of cutaneous BA (five cases) and BP of the liver (two cases) and spleen (one case). The blood isolates and BA and BP tissue samples were obtained from widely separated geographic areas. The antiserum was weakly cross-reactive with cultures of Rochalimaea quintana, an organism closely related to R. henselae, but this reactivity was eliminated by specific adsorption. The antiserum did not cross-react with the Warthin-Starry-positive organisms associated with cat scratch disease (Afipia felis), syphilis (Treponema pallidum), Lyme disease (Borrelia burgdorferi) or chronic active gastritis (Helicobacter pylori). Likewise, the antiserum did not identify organisms in eight cases of Kaposi's sarcoma, a disorder of immunocompromised patients that is clinically similar to BA. Further studies are needed to determine the prevalence of this newly recognized organism as well as its possible involvement in other angioproliferative diseases.",
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