Immunocytochemical localization of concanavalin A in developing jack-bean cotyledons

Eliot M Herman, Leland M. Shannon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The lectin, concanavalin A (Con A), was localized in the cotyledon of developing jack beans (Canavalia ensiformis (L.) DC) by electron-microscope immunocytochemistry. In mature seeds, Con A was present in protein-storage vacuoles (protein bodies) of storage-parenchyma cells. Although protein bodies could be seen in other cell types, only protein bodies in storage-parenchyma cells contained Con A. During seed development, Con A was also localized on the endoplasmic reticulum and Golgi apparatus, presumably en route toward deposition within the protein bodies. The intensity of labeling of the endoplasmic reticulum was much greater during the developmental stage of protein-body filling (66% final seed weight) than in mature seeds.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalPlanta
Volume161
Issue number2
DOIs
StatePublished - May 1984
Externally publishedYes

Fingerprint

Canavalia ensiformis
protein bodies
Cotyledon
concanavalin A
Concanavalin A
cotyledons
Seeds
Proteins
endoplasmic reticulum
Endoplasmic Reticulum
seeds
Canavalia
immunocytochemistry
cells
electron microscopes
storage proteins
Golgi apparatus
seed development
lectins
Golgi Apparatus

Keywords

  • Canavalia
  • Concanavalin A
  • Endoplasmic reticulum
  • Golgi apparatus
  • Lectin
  • Protein body

ASJC Scopus subject areas

  • Plant Science

Cite this

Immunocytochemical localization of concanavalin A in developing jack-bean cotyledons. / Herman, Eliot M; Shannon, Leland M.

In: Planta, Vol. 161, No. 2, 05.1984, p. 97-104.

Research output: Contribution to journalArticle

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