Immunocytological localization of 1,25-dihydroxyvitamin D3-like molecules and their receptors in a calcium-transporting epithelium of a crustacean

J. C. Meyran, G. Morel, M. R. Haussler, G. Boivin

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

For the first time, immunoreactivity to 1,25(OH)2D3-like molecules and their receptors has been investigated in a calcium transporting epithelium of a crustacean, Orchestia, using vertebrate antisera on ultrathin cryosections of posterior caeca previously fixed in glutaraldehyde, then postfixed in osmium tetroxide. Both immunoreactivities were located mainly in the nuclei of epithelial cells. Quantitative differences in 1,25(OH)2D3-like immunoreactivity were noticed from one stage of the molt cycle to another. These results, together with other data, contribute to evidence that immunoreactive 1,25(OH)2D3-like molecules may be involved in the regulatory processes of calcium metabolism in this terrestrial crustacean and suggest an involvement of these substances in the regulation of calcium movements in the posterior caeca.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalCell and Tissue Research
Volume263
Issue number2
DOIs
StatePublished - Feb 1991

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Keywords

  • 1,25(OH)D receptor-like immunoreactivity
  • Calcium ions
  • Immunocytochemistry
  • Orchestia cavimana (Crustacea)
  • Vitamin D-1,25(OH)D-like immunoreactivity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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