Immunofluorescence analysis of antisense oligodeoxynucleotide-mediated 'knock-down' of the mouse δ opioid receptor in vitro and in vivo

Josephine Lai, Maureen Riedl, Laura S. Stone, Ulf Arvidsson, Edward J. Bilsky, George L. Wilcox, Robert Elde, Frank Porreca

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We have previously used antisense oligodeoxynucleotides (ODN) to the cloned δ opioid receptor (DOR) to inhibit the antinociceptive response to spinally administered δ opioid receptor selective agonists in mice. Here we have examined the effect of DOR antisense ODN treatment on the level of DOR expressed in NG 108-15 cells and the spinal cord, through immune-fluorescence microscopy, to determine the efficiency and selectivity of the antisense ODN-mediated 'knock-down' of the DOR in these tissues. Antisense ODN, but not mismatch control, treatment resulted in a significant reduction in DOR immunoreactivity (-ir) in NG 108-15 cells and spinal cord. Thus, the inhibition of antinociceptive response to intrathecal δ selective agonists by DOR antisense ODN correlates with the loss of DOR-ir in the superficial layers of the dorsal horn of the spinal cord.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalNeuroscience Letters
Volume213
Issue number3
DOIs
StatePublished - Aug 9 1996

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Keywords

  • Antinociception
  • Antisense oligodeoxynucleotides
  • Cloned detla opioid receptor
  • Immunofluorescence microscopy
  • Mice cord

ASJC Scopus subject areas

  • Neuroscience(all)

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