Immunohistochemical analysis of cathepsin D in prostate carcinoma.

R. Makar, A. Mason, J. M. Kittelson, G. T. Bowden, Anne E Cress, Raymond B Nagle

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cathepsin D is a carboxyl protease that has been implicated as an important factor in tumor cell invasion. Sixty-nine cases of primary adenocarcinoma of the prostate were studied by the indirect immunoperoxidase method using a primary monoclonal anti-cathepsin D antibody. Immunoreactivity was graded from 0 (negative) to 4+ (intense reaction). The normal tubuloalveolar glands were, in general, negative. However, nine cases revealed focal staining of nonneoplastic luminal cells. Basal cells were negative except in areas of basal-cell hyperplasia, which were intensely positive. Thirty-nine of 78 carcinoma samples revealed 2+ or greater positive punctate lysosomal staining. In the 39 positive-stained cases, the reactivity was diffuse in three and focal in the remainder. The percentage of carcinoma cases whose worst lesions stained 2+ or greater showed a nonsignificant (P = 0.055) relation to Gleason grade but a significant (P = 0.031) relationship to pathologic stage. Thus, cathepsin D may prove to be a useful marker of prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)747-751
Number of pages5
JournalModern Pathology
Volume7
Issue number7
StatePublished - Sep 1994

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Cathepsin D
Prostate
Carcinoma
Staining and Labeling
Hyperplasia
Prostatic Neoplasms
Adenocarcinoma
Peptide Hydrolases
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Makar, R., Mason, A., Kittelson, J. M., Bowden, G. T., Cress, A. E., & Nagle, R. B. (1994). Immunohistochemical analysis of cathepsin D in prostate carcinoma. Modern Pathology, 7(7), 747-751.

Immunohistochemical analysis of cathepsin D in prostate carcinoma. / Makar, R.; Mason, A.; Kittelson, J. M.; Bowden, G. T.; Cress, Anne E; Nagle, Raymond B.

In: Modern Pathology, Vol. 7, No. 7, 09.1994, p. 747-751.

Research output: Contribution to journalArticle

Makar, R, Mason, A, Kittelson, JM, Bowden, GT, Cress, AE & Nagle, RB 1994, 'Immunohistochemical analysis of cathepsin D in prostate carcinoma.', Modern Pathology, vol. 7, no. 7, pp. 747-751.
Makar R, Mason A, Kittelson JM, Bowden GT, Cress AE, Nagle RB. Immunohistochemical analysis of cathepsin D in prostate carcinoma. Modern Pathology. 1994 Sep;7(7):747-751.
Makar, R. ; Mason, A. ; Kittelson, J. M. ; Bowden, G. T. ; Cress, Anne E ; Nagle, Raymond B. / Immunohistochemical analysis of cathepsin D in prostate carcinoma. In: Modern Pathology. 1994 ; Vol. 7, No. 7. pp. 747-751.
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