Immunohistochemical comparison of vascular and sinusoidal adherens junctions in cavernosal endothelium

Hunter Wessells, Stephen H. King, Monika Schmelz, Raymond B Nagle, Ronald L Heimark

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives. To characterize endothelial cell-to-cell junctions in the sinusoids and microvasculature of the corpus cavernosum. Methods. Corporal tissue was obtained from 6 potent human subjects, cut into 5-μm cryosections, and double-labeled with consecutive applications of primary and secondary antibodies. Laser scanning confocal microscopy identified subcellular localization of endothelial anchoring and adhesion molecules. Fluorescence intensity was rated as strong, weak, or absent by two observers. Results. The cavernosal endothelial adherens junction was composed of vascular endothelial cadherin, alpha-catenin, plakoglobin, vinculin, and the regulatory proteins beta-catenin and ZO-1. Adherens junctions in sinusoids were elongated, redundant, and narrow versus short, dense, linear cell-to-cell contacts in small arterioles and venules. Vinculin expression along the basal interface of the endothelium and stroma was weak in the sinusoids and strong in the arterioles. Definite sinusoidal expression of CD31 and CD34 was noted. P-selectin was only expressed within the cavernosal microvessels. Conclusions. The regulatory and structural proteins extending from vascular endothelial cadherin provide immunohistochemical evidence of a role for adherens junctions in cavernosal endothelial barrier function and cellular homeostasis. The sinusoidal endothelium has a unique junctional phenotype consistent with its blood trapping function. Differential expression of functional proteins in sinusoidal and microvascular endothelium may reflect segmental variation in hemodynamic exposure to pressure, stretch, or flow.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalUrology
Volume63
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Adherens Junctions
Vinculin
Endothelium
Blood Vessels
Arterioles
Microvessels
gamma Catenin
alpha Catenin
Proteins
P-Selectin
Intercellular Junctions
Venules
beta Catenin
Confocal Microscopy
Homeostasis
Endothelial Cells
Fluorescence
Hemodynamics
Phenotype
Pressure

ASJC Scopus subject areas

  • Urology

Cite this

Immunohistochemical comparison of vascular and sinusoidal adherens junctions in cavernosal endothelium. / Wessells, Hunter; King, Stephen H.; Schmelz, Monika; Nagle, Raymond B; Heimark, Ronald L.

In: Urology, Vol. 63, No. 1, 01.2004, p. 201-206.

Research output: Contribution to journalArticle

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abstract = "Objectives. To characterize endothelial cell-to-cell junctions in the sinusoids and microvasculature of the corpus cavernosum. Methods. Corporal tissue was obtained from 6 potent human subjects, cut into 5-μm cryosections, and double-labeled with consecutive applications of primary and secondary antibodies. Laser scanning confocal microscopy identified subcellular localization of endothelial anchoring and adhesion molecules. Fluorescence intensity was rated as strong, weak, or absent by two observers. Results. The cavernosal endothelial adherens junction was composed of vascular endothelial cadherin, alpha-catenin, plakoglobin, vinculin, and the regulatory proteins beta-catenin and ZO-1. Adherens junctions in sinusoids were elongated, redundant, and narrow versus short, dense, linear cell-to-cell contacts in small arterioles and venules. Vinculin expression along the basal interface of the endothelium and stroma was weak in the sinusoids and strong in the arterioles. Definite sinusoidal expression of CD31 and CD34 was noted. P-selectin was only expressed within the cavernosal microvessels. Conclusions. The regulatory and structural proteins extending from vascular endothelial cadherin provide immunohistochemical evidence of a role for adherens junctions in cavernosal endothelial barrier function and cellular homeostasis. The sinusoidal endothelium has a unique junctional phenotype consistent with its blood trapping function. Differential expression of functional proteins in sinusoidal and microvascular endothelium may reflect segmental variation in hemodynamic exposure to pressure, stretch, or flow.",
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N2 - Objectives. To characterize endothelial cell-to-cell junctions in the sinusoids and microvasculature of the corpus cavernosum. Methods. Corporal tissue was obtained from 6 potent human subjects, cut into 5-μm cryosections, and double-labeled with consecutive applications of primary and secondary antibodies. Laser scanning confocal microscopy identified subcellular localization of endothelial anchoring and adhesion molecules. Fluorescence intensity was rated as strong, weak, or absent by two observers. Results. The cavernosal endothelial adherens junction was composed of vascular endothelial cadherin, alpha-catenin, plakoglobin, vinculin, and the regulatory proteins beta-catenin and ZO-1. Adherens junctions in sinusoids were elongated, redundant, and narrow versus short, dense, linear cell-to-cell contacts in small arterioles and venules. Vinculin expression along the basal interface of the endothelium and stroma was weak in the sinusoids and strong in the arterioles. Definite sinusoidal expression of CD31 and CD34 was noted. P-selectin was only expressed within the cavernosal microvessels. Conclusions. The regulatory and structural proteins extending from vascular endothelial cadherin provide immunohistochemical evidence of a role for adherens junctions in cavernosal endothelial barrier function and cellular homeostasis. The sinusoidal endothelium has a unique junctional phenotype consistent with its blood trapping function. Differential expression of functional proteins in sinusoidal and microvascular endothelium may reflect segmental variation in hemodynamic exposure to pressure, stretch, or flow.

AB - Objectives. To characterize endothelial cell-to-cell junctions in the sinusoids and microvasculature of the corpus cavernosum. Methods. Corporal tissue was obtained from 6 potent human subjects, cut into 5-μm cryosections, and double-labeled with consecutive applications of primary and secondary antibodies. Laser scanning confocal microscopy identified subcellular localization of endothelial anchoring and adhesion molecules. Fluorescence intensity was rated as strong, weak, or absent by two observers. Results. The cavernosal endothelial adherens junction was composed of vascular endothelial cadherin, alpha-catenin, plakoglobin, vinculin, and the regulatory proteins beta-catenin and ZO-1. Adherens junctions in sinusoids were elongated, redundant, and narrow versus short, dense, linear cell-to-cell contacts in small arterioles and venules. Vinculin expression along the basal interface of the endothelium and stroma was weak in the sinusoids and strong in the arterioles. Definite sinusoidal expression of CD31 and CD34 was noted. P-selectin was only expressed within the cavernosal microvessels. Conclusions. The regulatory and structural proteins extending from vascular endothelial cadherin provide immunohistochemical evidence of a role for adherens junctions in cavernosal endothelial barrier function and cellular homeostasis. The sinusoidal endothelium has a unique junctional phenotype consistent with its blood trapping function. Differential expression of functional proteins in sinusoidal and microvascular endothelium may reflect segmental variation in hemodynamic exposure to pressure, stretch, or flow.

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