Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods

R. B. Nagle; V. A. Clark; K. M. McDaniel; J. R. Davis

doi:10.1177/31.8.6190854

Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods

R. B. Nagle, V. A. Clark, K. M. McDaniel, J. R. Davis

Cancer Center

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Abstract

A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

Original language	English (US)
Pages (from-to)	1010-1014
Number of pages	5
Journal	Journal of Histochemistry and Cytochemistry
Volume	31
Issue number	8
DOIs	https://doi.org/10.1177/31.8.6190854
State	Published - 1983

ASJC Scopus subject areas

Anatomy
Histology

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title = "Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods",

abstract = "A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.",

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AU - Nagle, R. B.

AU - Clark, V. A.

AU - McDaniel, K. M.

AU - Davis, J. R.

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N2 - A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

AB - A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

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