Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods

Raymond B Nagle, V. A. Clark, K. M. McDaniel, J. R. Davis

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Abstract

A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

Original languageEnglish (US)
Pages (from-to)1010-1014
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume31
Issue number8
Publication statusPublished - 1983

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ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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