Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods

R. B. Nagle, V. A. Clark, K. M. McDaniel, J. R. Davis

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A comparison of five immunohistochemical methods for the demonstration of keratins in humans ovarian neoplasms using affinity-purified polyclonal rabbit antibody was made. The use of indirect immunofluorescence on frozen sections briefly fixed in acetone was found to be the most sensitive method and demonstrated keratin in all 14 primary and 1 metastatic ovarian epithelial neoplasms studied. Protein A-peroxidase, peroxidase-antiperoxidase (PAP), indirect peroxidase, or the avidin-biotinylated peroxidase complex (ABC) methods applied to formalin-fixed tissues were less sensitive and led to false negative results in 9 of 15, 1 of 15, 8 of 15, and 6 of 15 cases, respectively. A single case of dysgerminoma failed to reveal keratin by any method.

Original languageEnglish (US)
Pages (from-to)1010-1014
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume31
Issue number8
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Anatomy
  • Histology

Fingerprint Dive into the research topics of 'Immunohistochemical demonstration of keratins in human ovarian neoplasms. A comparison of methods'. Together they form a unique fingerprint.

  • Cite this