Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts

Annette M. Sysel, Victor E. Valli, Raymond B Nagle, Joseph A. Bauer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background/Aim: Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. Materials and Methods: Tissue microarray slides containing 34 xenograft tumor tissues were immunohistochemically stained using TCN2 (anti-TCII), CD320 (anti-TCII-R) and MIB-1 (anti-Ki-67) antibodies. Representatively stained areas of all slides were digitally imaged and protein expression was quantified using ImageJ software plugins. Results: All xenograft tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Correlation between TCII/TCII-R and Ki-67 expression was not significant in xenograft tissues. Conclusion: Proliferating cancer cells express measurable levels of TCII and TCII-R. Immunohistochemical quantification of these markers may be useful as a tool for detection of tumors, tailored selection of anti-tumor therapies and surveillance for evidence of recurrent disease.

Original languageEnglish (US)
Pages (from-to)4203-4212
Number of pages10
JournalAnticancer Research
Volume33
Issue number10
StatePublished - Oct 2013

Fingerprint

Cell Surface Receptors
Vitamin B 12
Heterografts
Carrier Proteins
Neoplasms
Transcobalamins
Proteins
Software
Antibodies

Keywords

  • Immunohistochemistry
  • Ki-67
  • Receptor
  • Transcobalamin II
  • Transport protein
  • Vitamin B12
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts. / Sysel, Annette M.; Valli, Victor E.; Nagle, Raymond B; Bauer, Joseph A.

In: Anticancer Research, Vol. 33, No. 10, 10.2013, p. 4203-4212.

Research output: Contribution to journalArticle

@article{162deb61c95241a4a8838294205f6239,
title = "Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts",
abstract = "Background/Aim: Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. Materials and Methods: Tissue microarray slides containing 34 xenograft tumor tissues were immunohistochemically stained using TCN2 (anti-TCII), CD320 (anti-TCII-R) and MIB-1 (anti-Ki-67) antibodies. Representatively stained areas of all slides were digitally imaged and protein expression was quantified using ImageJ software plugins. Results: All xenograft tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Correlation between TCII/TCII-R and Ki-67 expression was not significant in xenograft tissues. Conclusion: Proliferating cancer cells express measurable levels of TCII and TCII-R. Immunohistochemical quantification of these markers may be useful as a tool for detection of tumors, tailored selection of anti-tumor therapies and surveillance for evidence of recurrent disease.",
keywords = "Immunohistochemistry, Ki-67, Receptor, Transcobalamin II, Transport protein, Vitamin B12, Xenograft",
author = "Sysel, {Annette M.} and Valli, {Victor E.} and Nagle, {Raymond B} and Bauer, {Joseph A.}",
year = "2013",
month = "10",
language = "English (US)",
volume = "33",
pages = "4203--4212",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "10",

}

TY - JOUR

T1 - Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts

AU - Sysel, Annette M.

AU - Valli, Victor E.

AU - Nagle, Raymond B

AU - Bauer, Joseph A.

PY - 2013/10

Y1 - 2013/10

N2 - Background/Aim: Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. Materials and Methods: Tissue microarray slides containing 34 xenograft tumor tissues were immunohistochemically stained using TCN2 (anti-TCII), CD320 (anti-TCII-R) and MIB-1 (anti-Ki-67) antibodies. Representatively stained areas of all slides were digitally imaged and protein expression was quantified using ImageJ software plugins. Results: All xenograft tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Correlation between TCII/TCII-R and Ki-67 expression was not significant in xenograft tissues. Conclusion: Proliferating cancer cells express measurable levels of TCII and TCII-R. Immunohistochemical quantification of these markers may be useful as a tool for detection of tumors, tailored selection of anti-tumor therapies and surveillance for evidence of recurrent disease.

AB - Background/Aim: Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. Materials and Methods: Tissue microarray slides containing 34 xenograft tumor tissues were immunohistochemically stained using TCN2 (anti-TCII), CD320 (anti-TCII-R) and MIB-1 (anti-Ki-67) antibodies. Representatively stained areas of all slides were digitally imaged and protein expression was quantified using ImageJ software plugins. Results: All xenograft tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Correlation between TCII/TCII-R and Ki-67 expression was not significant in xenograft tissues. Conclusion: Proliferating cancer cells express measurable levels of TCII and TCII-R. Immunohistochemical quantification of these markers may be useful as a tool for detection of tumors, tailored selection of anti-tumor therapies and surveillance for evidence of recurrent disease.

KW - Immunohistochemistry

KW - Ki-67

KW - Receptor

KW - Transcobalamin II

KW - Transport protein

KW - Vitamin B12

KW - Xenograft

UR - http://www.scopus.com/inward/record.url?scp=84891359260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891359260&partnerID=8YFLogxK

M3 - Article

C2 - 24122983

AN - SCOPUS:84891359260

VL - 33

SP - 4203

EP - 4212

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 10

ER -