Immunohistochemical quantification of the vitamin B12 transport protein (TCII), cell surface receptor (TCII-R) and Ki-67 in human tumor xenografts

Annette M. Sysel, Victor E. Valli, Raymond B Nagle, Joseph A. Bauer

Research output: Contribution to journalArticle

22 Scopus citations


Background/Aim: Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. Materials and Methods: Tissue microarray slides containing 34 xenograft tumor tissues were immunohistochemically stained using TCN2 (anti-TCII), CD320 (anti-TCII-R) and MIB-1 (anti-Ki-67) antibodies. Representatively stained areas of all slides were digitally imaged and protein expression was quantified using ImageJ software plugins. Results: All xenograft tumor tissues stained positively for TCII, TCII-R and Ki-67 proteins; expression varied both within and between tumor types. Correlation between TCII/TCII-R and Ki-67 expression was not significant in xenograft tissues. Conclusion: Proliferating cancer cells express measurable levels of TCII and TCII-R. Immunohistochemical quantification of these markers may be useful as a tool for detection of tumors, tailored selection of anti-tumor therapies and surveillance for evidence of recurrent disease.

Original languageEnglish (US)
Pages (from-to)4203-4212
Number of pages10
JournalAnticancer Research
Issue number10
Publication statusPublished - Oct 2013



  • Immunohistochemistry
  • Ki-67
  • Receptor
  • Transcobalamin II
  • Transport protein
  • Vitamin B12
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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